The Expression Characteristics of Transcription Factors Regulated by Exogenous ABA in Winter Wheat (Triticum aestivum) under Cold Stress

被引：9

作者：

Lv, Y. ^{[1
]}

Song, C. H. ^{[1
]}

Lu, Q. W. ^{[1
]}

Tian, Y. ^{[1
]}

Li, H. D. ^{[1
]}

Zhang, D. ^{[1
]}

Yu, J. ^{[1
]}

Xu, Q. H. ^{[1
]}

Cang, J. ^{[1
]}

机构：

[1] Northeast Agr Univ, Coll Life Sci, Harbin 150030, Heilongjiang, Peoples R China

来源：

RUSSIAN JOURNAL OF PLANT PHYSIOLOGY | 2018年 / 65卷 / 06期

关键词：

Triticum aestivum; winter wheat; exogenous ABA; cold-stress; transcription factors; subcellular localization; ABSCISIC-ACID; BINDING PROTEINS; LEUCINE-ZIPPER; DNA-BINDING; ARABIDOPSIS; SEQUENCE; HVABI5; GENOME;

D O I：

10.1134/S1021443718060134

中图分类号：

Q94 [植物学];

学科分类号：

071001 ;

摘要：

Dongnong Dongmai1 (Dn1)' is a cultivar of wheat (Triticum aestivum L.) which may endure30 degrees C in field with a reviving rate of 85%. ABA may enhance plant resistance. Aiming to reveal Dn1 coldresistant mechanism after exogenous ABA (exo-ABA) treated (control: non-ABA treated), some transcription factors (TFs) were selected and detected. Seedlings were prayed with exo-ABA at three-leaf stage, subsequently their tilling nodes were respectively sampled when it was at 5, 0,-10 and-25 degrees C in field. Six TF genes (TabZIP1, TaWABI5, TaMYB1, TaMYB80, TaNAC2 and TaWRKY80) were selected and their expression at different low temperature was detected, the results showed that: in non-ABA treated tilling nodes, the expression of six TF genes all reached their peak level at-10 degrees C; in exo-ABA treated tilling nodes, the expression of five TF genes (TabZIP1, TaWABI5, TaMYB80, TaNAC2 and TaWRKY80) reached their peak level at-25 degrees C, and particularly the expression level of TabZIP1 was the highest in five TFs. In order to further defined the biological function of TabZIP1, the physical and chemical properties, protein secondary structure and the evolutionary tree was analyzed and predicted by some bioinformatics software or online program; TabZIP1 was localized in nuclear of tobacco (Nicotiana tabacum), which was observed by laser scanning confocal microscope (LSCM). This study initially exposed some TFs in Dn1 were upregulated to respond to the coldstress after exo-ABA treated, which indicated that a serious cold-responsive genes could be transcripted with the TFs assistance, and the results provided new theoretical basis to reveal the cold-reponsive mechanism of winter wheat.

引用

页码：842 / 848

页数：7

共 24 条

[1]

Cang Jing Cang Jing, 2012, Journal of Northeast Agricultural University (English Edition), V19, P27

[2] The transcription factors HvABI5 and HvVP1 are required for the abscisic acid induction of gene expression in barley aleurone cells [J].

Casaretto, J ;

Ho, THD .

PLANT CELL, 2003, 15 (01) :271-284

[3] Identification of Interactions between Abscisic Acid and Ribulose-1,5-Bisphosphate Carboxylase/Oxygenase [J].

Galka, Marek M. ;

Rajagopalan, Nandhakishore ;

Buhrow, Leann M. ;

Nelson, Ken M. ;

Switala, Jacek ;

Cutler, Adrian J. ;

Palmer, David R. J. ;

Loewen, Peter C. ;

Abrams, Suzanne R. ;

Loewen, Michele C. .

PLOS ONE, 2015, 10 (07)

[4] The Arabidopsis B-BOX Protein BBX25 Interacts with HY5, Negatively Regulating BBX22 Expression to Suppress Seedling Photomorphogenesis [J].

Gangappa, Sreeramaiah N. ;

Crocco, Carlos D. ;

Johansson, Henrik ;

Datta, Sourav ;

Hettiarachchi, Chamari ;

Holm, Magnus ;

Botto, Javier F. .

PLANT CELL, 2013, 25 (04) :1243-1257

[5] The Arabidopsis bZIP1 Transcription Factor Is Involved in Sugar Signaling, Protein Networking, and DNA Binding [J].

Kang, Shin Gene ;

Price, John ;

Lin, Pei-Chi ;

Hong, Jong Chan ;

Jang, Jyan-Chyun .

MOLECULAR PLANT, 2010, 3 (02) :361-373

[6] Analysis of the genome sequence of the flowering plant Arabidopsis thaliana [J].

Kaul, S ;

Koo, HL ;

Jenkins, J ;

Rizzo, M ;

Rooney, T ;

Tallon, LJ ;

Feldblyum, T ;

Nierman, W ;

Benito, MI ;

Lin, XY ;

Town, CD ;

Venter, JC ;

Fraser, CM ;

Tabata, S ;

Nakamura, Y ;

Kaneko, T ;

Sato, S ;

Asamizu, E ;

Kato, T ;

Kotani, H ;

Sasamoto, S ;

Ecker, JR ;

Theologis, A ;

Federspiel, NA ;

Palm, CJ ;

Osborne, BI ;

Shinn, P ;

Conway, AB ;

Vysotskaia, VS ;

Dewar, K ;

Conn, L ;

Lenz, CA ;

Kim, CJ ;

Hansen, NF ;

Liu, SX ;

Buehler, E ;

Altafi, H ;

Sakano, H ;

Dunn, P ;

Lam, B ;

Pham, PK ;

Chao, Q ;

Nguyen, M ;

Yu, GX ;

Chen, HM ;

Southwick, A ;

Lee, JM ;

Miranda, M ;

Toriumi, MJ ;

Davis, RW .

NATURE, 2000, 408 (6814) :796-815

[7] Positive role of a wheat HvABI5 ortholog in abiotic stress response of seedlings [J].

Kobayashi, Fuminori ;

Maeta, Eri ;

Terashima, Akihiro ;

Takumi, Shigeo .

PHYSIOLOGIA PLANTARUM, 2008, 134 (01) :74-86

[8] Role of CBFs as Integrators of Chloroplast Redox, Phytochrome and Plant Hormone Signaling during Cold Acclimation [J].

Kurepin, Leonid V. ;

Dahal, Keshav P. ;

Savitch, Leonid V. ;

Singh, Jas ;

Bode, Rainer ;

Ivanov, Alexander G. ;

Hurry, Vaughan ;

Huener, Norman P. A. .

INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES, 2013, 14 (06) :12729-12763

[9] THE LEUCINE ZIPPER - A HYPOTHETICAL STRUCTURE COMMON TO A NEW CLASS OF DNA-BINDING PROTEINS [J].

LANDSCHULZ, WH ;

JOHNSON, PF ;

MCKNIGHT, SL .

SCIENCE, 1988, 240 (4860) :1759-1764

[10] In vivo binding of hot pepper bZIP transcription factor CabZIP1 to the G-box region of pathogenesis-related protein 1 promoter [J].

Lee, BJ ;

Park, CJ ;

Kim, SK ;

Kim, KJ ;

Paek, KH .

BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS, 2006, 344 (01) :55-62

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