Optical anisotropies in corneal stroma collagen fibers from diabetic spontaneous mice

被引：17
作者：
Aldrovani, M. [1 ]
Guaraldo, A. M. A. [2 ]
Vidal, B. C. [1 ]
机构：
[1] Univ Estadual Campinas, Inst Biol, Dept Cell Biol, BR-13083863 Campinas, SP, Brazil
[2] Univ Estadual Campinas, Multidisciplinary Ctr Biol Invest, BR-13083863 Campinas, SP, Brazil
来源：
VISION RESEARCH | 2007年 / 47卷 / 26期
基金：
巴西圣保罗研究基金会;
关键词：
birefringence; collagen; cornea; diabetes; dichroism;
D O I：
10.1016/j.visres.2007.02.011
中图分类号：
Q189 [神经科学];
学科分类号：
071006 ;
摘要：
In this work, optical retardation (OR) was investigated in corneal stroma collagen fibers from nonobese diabetic (NOD) and healthy (BALB/c) mice. Linear dichroism (LD) was investigated in corneas stained with Ponceau SS and Toluidine Blue, using microspectrophotometer. NOD corneas showed higher OR values than the control. Ponceau SS-complexed collagen fibers showed positive LD that was higher for NOD corneas. After staining with Toluidine Blue, NOD and BALB/c corneas showed negative LD. The results of this study demonstrate that diabetes was capable of altering the corneal optical anisotropies, possibly altering the number of intermolecular crosslinks in collagen fibers. (c) 2007 Elsevier Ltd. All rights reserved.
引用
收藏
页码：3229 / 3237
页数：9
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