Tamoxifen-inducible Cre-recombination in articular chondrocytes of adult Col2a1-CreERT2 transgenic mice

被引：44

作者：

Zhu, M. ^{[1
]}

Chen, M. ^{[1
]}

Lichtler, A. C. ^{[2
]}

O'Keefe, R. J. ^{[1
]}

Chen, D. ^{[1
]}

机构：

[1] Univ Rochester, Sch Med, Dept Orthopaed, Ctr Musculoskeletal Res, Rochester, NY 14642 USA

[2] Univ Connecticut, Ctr Hlth, Dept Genet & Dev Biol, Farmington, CT USA

来源：

OSTEOARTHRITIS AND CARTILAGE | 2008年 / 16卷 / 01期

关键词：

articular chondrocyte; tamoxifen; Cre-recombination;

D O I：

10.1016/j.joca.2007.08.001

中图分类号：

R826.8 [整形外科学]; R782.2 [口腔颌面部整形外科学]; R726.2 [小儿整形外科学]; R62 [整形外科学（修复外科学）];

学科分类号：

摘要：

Objective: To determine the specificity and efficiency of the tamoxifen (TM)-induced Cre-recombination in articular chondrocytes of adult Col2a1-CreER(T2) transgenic mice. Methods: Col2a1-CreER(T2) transgenic mice were bred with Rosa26 reporter mice. Two-week-old Col2a1-CreER(T2);R26R mice were administered TM for 5 days and were sacrificed 1 and 6 months after TM induction. X-Gal staining was performed. Results: Efficient Cre-recombination is achieved in adult articular chondrocytes 1 and 6 months after TM induction. Conclusion: Our findings demonstrate that the Col2a1-CreER(T2) transgenic mouse model is a valuable tool to target genes specifically expressed in articular chondrocytes in a temporally controlled manner in adult mice. (c) 2007 Osteoarthritis Research Society International. Published by Elsevier Ltd. All rights reserved.

引用

页码：129 / 130

页数：2

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