COMPARISON OF TWO AVAILABLE PLATFORMS FOR DETERMINATION OF RNA QUALITY

被引：17

作者：

Riedmaier, I. ^{[1
]}

Bergmaier, M. ^{[1
]}

Pfaffl, M. W. ^{[1
]}

机构：

[1] Tech Univ Munich, D-8050 Freising Weihenstephan, Germany

来源：

BIOTECHNOLOGY & BIOTECHNOLOGICAL EQUIPMENT | 2010年 / 24卷 / 04期

关键词：

RNA quality; RNA integrity; RNA quantity; lab-on-chip; DEGRADATION; IMPACT;

D O I：

10.2478/V10133-010-0074-7

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

The integrity of RNA is a very critical aspect regarding downstream RNA based quantitative analysis like RT-qPCR Low-quality RNA can compromise the results of such experiments Today automated lab-on-chip capillary electrophoresis allows rapid RNA quality and quantity determination e g 2100 Bioanalyzer (Agilent Technologies) and the Experion (Bio-Rad) Both platforms determine RNA quality using a numerical system which represents the integrity of RNA The Bioanalyzer offers the RIN algorithm (RNA Integrity Number) on the Bioanalyzer 2100 and Bio-Rad developed a new Experion software version that offers an algorithm for calculating the RNA Quality Index (RQI) The aim of this study was to compare both systems regarding sensitivity reproducibility linearity and the influence of individual tissue extractions and different chip runs on RNA quality and quantity determination Overall it was confirmed that both algorithms are very comparable and beneficial for the determination of RNA quality for downstream applications The Experion showed slightly better results regarding reproducibility and absolute sensitivity whereas the 2100 Bioanalyzer showed a higher linearity

引用

页码：2154 / 2159

页数：6

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