A seven-gene-deleted African swine fever virus is safe and effective as a live attenuated vaccine in pigs

被引：242

作者：

Chen, Weiye ^{[1
]}

Zhao, Dongming ^{[1
]}

He, Xijun ^{[2
]}

Liu, Renqiang ^{[1
]}

Wang, Zilong ^{[1
]}

Zhang, Xianfeng ^{[2
]}

Li, Fang ^{[1
]}

Shan, Dan ^{[1
]}

Chen, Hefeng ^{[1
]}

Zhang, Jiwen ^{[1
]}

Wang, Lulu ^{[1
]}

Wen, Zhiyuan ^{[1
]}

Wang, Xijun ^{[1
]}

Guan, Yuntao ^{[2
]}

Liu, Jinxiong ^{[1
]}

Bu, Zhigao ^{[1
,2
]}

机构：

[1] Chinese Acad Agr Sci, Harbin Vet Res Inst, State Key Lab Vet Biotechnol, Harbin 150001, Peoples R China

[2] Natl High Containment Lab Anim Dis Control & Prev, Harbin 150069, Peoples R China

来源：

SCIENCE CHINA-LIFE SCIENCES | 2020年 / 63卷 / 05期

基金：

国家重点研发计划;

关键词：

African swine fever; vaccine; safety; protective efficacy; pig; GENETIC-CHARACTERIZATION; DOMESTIC PIGS; VIRULENCE; DELETION; GEORGIA; ISOLATE; GENOME; CHINA; TRANSMISSION; PROTECTION;

D O I：

10.1007/s11427-020-1657-9

中图分类号：

Q [生物科学];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

African swine fever (ASF) is a devastating infectious disease in swine that is severely threatening the global pig industry. An efficacious vaccine is urgently required. Here, we used the Chinese ASFV HLJ/18 as a backbone and generated a series of gene-deleted viruses. The virulence, immunogenicity, safety, and protective efficacy evaluation in specific-pathogen-free pigs, commercial pigs, and pregnant sows indicated that one virus, namely HLJ/18-7GD, which has seven genes deleted, is fully attenuated in pigs, cannot convert to the virulent strain, and provides complete protection of pigs against lethal ASFV challenge. Our study shows that HLJ/-18-7GD is a safe and effective vaccine against ASFV, and as such is expected to play an important role in controlling the spread of ASFV.

引用

页码：623 / 634

页数：12

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