NeuN immunoreactivity in the brain of Xenopus laevis

被引：6

作者：

Saito, Shouichiro ^{[1
]}

Shiina, Takahiko ^{[2
]}

Shimizu, Yasutake ^{[2
]}

机构：

[1] Gifu Univ, Lab Vet Anat, United Grad Sch Vet Sci, 1-1 Yanagido, Gifu 5011193, Japan

[2] Gifu Univ, Lab Vet Physiol, United Grad Sch Vet Sci, 1-1 Yanagido, Gifu 5011193, Japan

来源：

TISSUE & CELL | 2017年 / 49卷 / 04期

关键词：

Antigen retrieval; Fox-3; Phylogeny; FIBRILLARY ACIDIC PROTEIN; NUCLEAR ANTIGEN NEUN; NEURONAL MARKER NEUN; DIFFERENTIATION; VERTEBRATES; EXPRESSION; MIGRATION; NEUROGLIA;

D O I：

10.1016/j.tice.2017.05.001

中图分类号：

R602 [外科病理学、解剖学]; R32 [人体形态学];

学科分类号：

100101 ;

摘要：

Neuronal nuclear antigen (NeuN), discovered in mice brain cell nuclei by Mullen et al. (1992), is used as an excellent marker of post-mitotic neurons in vertebrates. In this study, the expression pattern of NeuN was examined in the Xenopus brain to explore phylogenetic differences in NeuN expression. Anti-NeuN antibody showed selective staining in mouse and Xenopus brain extracts, but the number and molecular weight of the bands differed in Western blotting analysis. In immunostaining, anti-NeuN antibody showed selective staining of neurons, but not glial cells, in the Xenopus brain. Most neurons, including olfactory bulb mitral cells and cerebellar Purkinjie cells, which show no immunoreactivity in birds/mammals, showed NeuN immunoreactivity in Xenopus. This study revealed that anti-NeuN antibody is a useful marker of post-mitotic neurons in amphibians, but it also stains neurons that show no reactivity in more derived animals. (C) 2017 Elsevier Ltd. All rights reserved.

引用

页码：514 / 519

页数：6

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