Thrombin Binding Aptamer, More than a Simple Aptamer: Chemically Modified Derivatives and Biomedical Applications

被引:115
作者
Avino, Anna [1 ]
Fabrega, Carme [1 ]
Tintore, Maria [1 ]
Eritja, Ramon [1 ]
机构
[1] IQAC CSIC, CIBER BBN Networking Ctr Bioengn Biomat & Nanomed, Inst Res Biomed, E-08028 Barcelona, Spain
关键词
Thrombin binding aptamer; TBA; G-quadruplex; thrombin; anticoagulant; oligonucleotide synthesis; DNA; aptamer; SINGLE-STRANDED-DNA; G-QUADRUPLEX FORMATION; G-C REPEATS; NUCLEIC-ACID; IN-VIVO; EXPONENTIAL ENRICHMENT; MOLECULAR RECOGNITION; SYSTEMATIC EVOLUTION; PROTEIN-DETECTION; HUMAN TELOMERE;
D O I
10.2174/138161212799958387
中图分类号
R9 [药学];
学科分类号
1007 ;
摘要
The thrombin binding aptamer (TBA) is a well characterized chair-like, antiparallel quadruplex structure that binds specifically to thrombin at nanomolar concentrations and therefore it has interesting anticoagulant properties. In this article we review the research involved in the development of new TBA derivatives with improved anticoagulant properties as well as the use of the TBA as a model compound for the study of quadruplex structures. Specifically, we describe the impact of modified nucleosides and non-natural backbones in the guanine tetrads or in the loops and the introduction of pendant groups at the 3' or 5' -ends. The modified oligonucleotides are shown to be excellent tools for the understanding of the molecular structure of the TBA and its folding properties. Finally, we review the use of the TBA-Thrombin recognition system for the development of analytical tools based on the TBA folding.
引用
收藏
页码:2036 / 2047
页数:12
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