Effects of nicotine on a rat model of early stage osteoarthritis

被引：0
作者：
Gu, Qiangrong [1 ,2 ]
Li, Dong [1 ,2 ]
Wei, Bo [1 ,2 ]
Guo, Yang [1 ]
Yan, Junwei [1 ]
Mao, Fengyong [1 ]
Zhang, Xiang [1 ]
Wang, Liming [1 ,2 ]
机构：
[1] Nanjing Med Univ, Dept Orthoped, Nanjing Hosp 1, Nanjing 210006, Jiangsu, Peoples R China
[2] Nanjing Med Univ, Cartilage Regenerat Ctr, Nanjing Hosp 1, Nanjing 210006, Jiangsu, Peoples R China
来源：
INTERNATIONAL JOURNAL OF CLINICAL AND EXPERIMENTAL PATHOLOGY | 2015年 / 8卷 / 04期
基金：
中国国家自然科学基金;
关键词：
Nicotine; cartilage degeneration; early stage OA; alpha; 7nAChR; T2; mapping; COLLAGEN-INDUCED ARTHRITIS; RECEPTOR ALPHA-7 SUBUNIT; RHEUMATOID-ARTHRITIS; ACETYLCHOLINE-RECEPTOR; ARTICULAR-CARTILAGE; SIGNALING PATHWAY; EXPRESSION; IMMOBILIZATION; REMOBILIZATION; STIMULATION;
D O I：
暂无
中图分类号：
R73 [肿瘤学];
学科分类号：
100214 ;
摘要：
The objective of the study was to investigate the effects of nicotine on articular cartilage degeneration and inflammation in a rat model of early stage osteoarthritis (OA), using T2 mapping. In this study, a rat model of early stage OA was established by immobilizing the left knee joints of adult male rats for two weeks. Subsequently, rats were fed with nicotine for two and four weeks. Changes in the articular cartilage from the medial femoral condylar region of the knee were evaluated by gross observation and histological grading with the contents of cartilage matrix detected. T2 values of the articular cartilage were estimated through high-field magnetic resonance imaging (MRI) (7.OT). Levels of serum tumor necrosis factor-alpha (TNF-alpha) were assessed by ELISA. The expression of TNF-alpha and the cholinergic receptor, alpha 7nAChR, in the synovial tissue was measured by RT-PCR. Nicotine treatment ameliorated cartilage destruction, promoted matrix production, reduced the serum level of TNF-alpha and the expression of TNF-alpha in the synovial tissue, and increased the expression of alpha 7nAChR in the synovial tissue in the rat model of early stage OA. In conclusion, nicotine prevented cartilage damage and had an anti-inflammatory effect in a rat model of early stage OA. Thus nicotine may have potential as a therapeutic strategy for early stage OA.
引用
收藏
页码：3602 / 3612
页数：11
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