Serial two-photon tomography for automated ex vivo mouse brain imaging

被引：1

作者：

Ragan, Timothy ^{[2
]}

Kadiri, Lolahon R. ^{[1
]}

Venkataraju, Kannan Umadevi ^{[1
]}

Bahlmann, Karsten ^{[2
]}

Sutin, Jason ^{[2
]}

Taranda, Julian ^{[1
]}

Arganda-Carreras, Ignacio ^{[3
]}

Kim, Yongsoo ^{[1
]}

Seung, H. Sebastian ^{[3
]}

Osten, Pavel ^{[1
]}

机构：

[1] Cold Spring Harbor Lab, Cold Spring Harbor, NY 11724 USA

[2] TissueVision Inc, Cambridge, MA USA

[3] MIT, Howard Hughes Med Inst, Dept Brain & Cognit Sci, Cambridge, MA USA

来源：

NATURE METHODS | 2012年 / 9卷 / 03期

基金：

美国国家卫生研究院;

关键词：

NERVOUS-SYSTEM; BARREL CORTEX; IN-VIVO; MICROSCOPY; RECONSTRUCTION; EXPRESSION; TISSUE; REGISTRATION; CIRCUITS; VECTORS;

D O I：

10.1038/NMETH.1854

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

Here we describe an automated method, named serial two-photon (STP) tomography, that achieves high-throughput fluorescence imaging of mouse brains by integrating two-photon microscopy and tissue sectioning. STP tomography generates high-resolution datasets that are free of distortions and can be readily warped in three dimensions, for example, for comparing multiple anatomical tracings. This method opens the door to routine systematic studies of neuroanatomy in mouse models of human brain disorders.

引用

页码：255 / U48

页数：7

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