In Situ Patterning of Microfluidic Networks in 3D Cell-Laden Hydrogels

被引：146
作者：
Brandenberg, Nathalie [1 ]
Lutolf, Matthias P. [1 ,2 ]
机构：
[1] Ecole Polytech Fed Lausanne, Sch Life Sci, Inst Bioengn, Lab Stem Cell Bioengn, CH-1015 Lausanne, Switzerland
[2] Ecole Polytech Fed Lausanne, Sch Basic Sci, Inst Chem Sci & Engn, CH-1015 Lausanne, Switzerland
来源：
ADVANCED MATERIALS | 2016年 / 28卷 / 34期
基金：
瑞士国家科学基金会;
关键词：
hydrogels; microfabrication; microfluidics; short-pulsed lasers; tissue engineering; FABRICATION; MATRIX; GROWTH; FATE;
D O I：
10.1002/adma.201601099
中图分类号：
O6 [化学];
学科分类号：
0703 ;
摘要：
Focalized short-pulsed lasers have sufficient power to generate micrometersized cavities in various hydrogels. An in situ technique based on laser ablation to fabricate intricate microfluidic networks in biocompatible gels without manual handling is presented. This method is fully compatible with 3D cell culture and opens up unprecedented opportunities for cell biology, developmental biology, and stem-cell-based tissue engineering.
引用
收藏
页码：7450 / 7456
页数：7
相关论文
共 32 条
  • [1] Laser-based three-dimensional multiscale micropatterning of biocompatible hydrogels for customized tissue engineering scaffolds
    Applegate, Matthew B.
    Coburn, Jeannine
    Partlow, Benjamin P.
    Moreau, Jodie E.
    Mondia, Jessica P.
    Marelli, Benedetto
    Kaplan, David L.
    Omenetto, Fiorenzo G.
    [J]. PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 2015, 112 (39) : 12052 - 12057
  • [2] Engineers are from PDMS-land, Biologists are from Polystyrenia
    Berthier, Erwin
    Young, Edmond W. K.
    Beebe, David
    [J]. LAB ON A CHIP, 2012, 12 (07) : 1224 - 1237
  • [3] Microfluidic organs-on-chips
    Bhatia, Sangeeta N.
    Ingber, Donald E.
    [J]. NATURE BIOTECHNOLOGY, 2014, 32 (08) : 760 - 772
  • [4] The meaning, the sense and the significance: translating the science of mesenchymal stem cells into medicine
    Bianco, Paolo
    Cao, Xu
    Frenette, Paul S.
    Mao, Jeremy J.
    Robey, Pamela G.
    Simmons, Paul J.
    Wang, Cun-Yu
    [J]. NATURE MEDICINE, 2013, 19 (01) : 35 - 42
  • [5] Microfluidic scaffolds for tissue engineering
    Choi, Nak Won
    Cabodi, Mario
    Held, Brittany
    Gleghorn, Jason P.
    Bonassar, Lawrence J.
    Stroock, Abraham D.
    [J]. NATURE MATERIALS, 2007, 6 (11) : 908 - 915
  • [6] DIFFUSION IN EMBRYOGENESIS
    CRICK, F
    [J]. NATURE, 1970, 225 (5231) : 420 - &
  • [7] DeForest CA, 2015, NAT MATER, V14, P523, DOI [10.1038/NMAT4219, 10.1038/nmat4219]
  • [8] Advances in Bioactive Hydrogels to Probe and Direct Cell Fate
    DeForest, Cole A.
    Anseth, Kristi S.
    [J]. ANNUAL REVIEW OF CHEMICAL AND BIOMOLECULAR ENGINEERING, VOL 3, 2012, 3 : 421 - 444
  • [9] Dvir T, 2011, NAT NANOTECHNOL, V6, P13, DOI [10.1038/nnano.2010.246, 10.1038/NNANO.2010.246]
  • [10] Elucidating the Role of Matrix Stiffness in 3D Cell Migration and Remodeling
    Ehrbar, M.
    Sala, A.
    Lienemann, P.
    Ranga, A.
    Mosiewicz, K.
    Bittermann, A.
    Rizzi, S. C.
    Weber, F. E.
    Lutolf, M. P.
    [J]. BIOPHYSICAL JOURNAL, 2011, 100 (02) : 284 - 293
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