Windup of Nociceptive Flexion Reflex Depends on Synaptic and Intrinsic Properties of Dorsal Horn Neurons in Adult Rats

被引:5
作者
Aby, Franck [1 ]
Bouali-Benazzouz, Rabia [1 ]
Landry, Marc [1 ]
Fossat, Pascal [1 ]
机构
[1] Univ Bordeaux, Dept Neurocampus, Inst Interdisciplinaire Neurosci, IINS,CNRS,UMR 5297, 146 Rue Leo Saignat, F-33077 Bordeaux, France
关键词
pain; central sensitization; L-type calcium channels; NMDA receptors; glycine; dorsal horn of the spinal cord; CALCIUM-CHANNELS; CENTRAL SENSITIZATION; TEMPORAL SUMMATION; NMDA RECEPTOR; SPINAL-CORD; PAIN; HYPERSENSITIVITY; POTENTIALS; MECHANISMS; STIMULI;
D O I
10.3390/ijms20246146
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
Windup, a progressive increase in spinal response to repetitive stimulations of nociceptive peripheral fibers, is a useful model to study central sensitization to pain. Windup is expressed by neurons in both the dorsal and ventral horn of the spinal cord. In juvenile rats, it has been demonstrated both in vivo and in vitro that windup depends on calcium-dependent intrinsic properties and their modulation by synaptic components. However, the involvement of these two components in the adults remains controversial. In the present study, by means of electromyographic and extracellular recordings, we show that windup in adults, in vivo, depends on a synaptic balance between excitatory N-methyl-D-aspartate (NMDA) receptors and inhibitory glycinergic receptors. We also demonstrate the involvement of L-type calcium channels in both the dorsal and ventral horn of the spinal cord. These results indicate that windup in adults is similar to juvenile rats and that windup properties are the same regardless of the spinal network, i.e., sensory or motor.
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页数:15
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