Primary chondrocytes enhance cartilage tissue formation upon co-culture with a range of cell types

被引:31
作者
Hendriks, Jeanine A. A. [1 ,2 ]
Miclea, Razvan L. [3 ]
Schotel, Roka [2 ]
de Bruijn, Ewart [2 ]
Moroni, Lorenzo [1 ]
Karperien, Marcel [1 ,4 ]
Riesle, Jens [2 ]
van Blitterswijk, Clemens A. [1 ]
机构
[1] Univ Twente, Inst Biomed Technol, NL-7500 AE Enschede, Netherlands
[2] CellCoTec, NL-3720 MB Bilthoven, Netherlands
[3] Dept Pediat LUMC, NL-2300 RC Leiden, Netherlands
[4] LUMC, Dept Endocrinol, NL-2300 RC Leiden, Netherlands
关键词
MESENCHYMAL STEM-CELLS; HUMAN ARTICULAR CHONDROCYTES; IN-VITRO; DERMAL FIBROBLASTS; DIFFERENTIATION; CULTURE; HEPATOCYTES; GROWTH; MODEL; CHONDROGENESIS;
D O I
10.1039/c0sm00266f
中图分类号
O64 [物理化学(理论化学)、化学物理学];
学科分类号
070304 ; 081704 ;
摘要
Co-culture models have been increasingly used in tissue engineering applications to understand cell-cell interactions and consequently improve regenerative medicine strategies. Aiming at further elucidating cartilage tissue formation, we co-cultured bovine primary chondrocytes (BPCs) with human expanded chondrocytes (HECs), human dermal fibroblasts (HDFs), mouse embryonic stem cells (MESCs), or mouse-3T3 feeder cells (M3T3s) in micromasses. BPCs were either co-cultured (1 : 5 ratio) with all cell types allowing direct cell-cell contacts or as separate micromasses in the same well with HECs. In co-culture groups with direct cell-cell contacts cartilaginous tissue was formed in all experimental groups. In situ hybridization showed that only 16-27% of the cells expressed type II collagen mRNA. Corresponding with the fact that micromasses consisted for approximately 20% only of BPCs, the amount of GAG was similar between 100% BPC micromass and the co-culture groups with HECs and HDFs. Therefore, co-culture micromasses support cartilage tissue formation predominantly originating from primary chondrocytes in direct contact with a variety of cell types. These findings potentially could be applied to optimize cell-therapy treatments for cartilage regeneration.
引用
收藏
页码:5080 / 5088
页数:9
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