Development of agro-infectious clones for screening resistance against recombinant mungbean yellow mosaic India virus causing golden mosaic disease in vegetable cowpea

被引：3

作者：

Krishnan, Nagendran ^{[1
]}

Reddy, B. Rajasekhar ^{[2
]}

Kumari, Shweta ^{[1
]}

Singh, Achuit Kumar ^{[2
]}

机构：

[1] ICAR Indian Inst Vegetable Res, Div Crop Protect, Varanasi 221305, Uttar Pradesh, India

[2] ICAR Indian Inst Vegetable Res, Div Crop Improvement, Varanasi 221305, Uttar Pradesh, India

来源：

3 BIOTECH | 2022年 / 12卷 / 07期

关键词：

Begomovirus; Legume viruses; Recombination; Agro-infectious clones; Cowpea; Agro-inoculation; PCR-MEDIATED AMPLIFICATION; VIGNA-MUNGO; MOLECULAR CHARACTERIZATION; UNIVERSAL PRIMERS; DNA-BETA; TRANSMISSION; ISOLATE;

D O I：

10.1007/s13205-022-03206-2

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

Begomovirus associated with golden mosaic disease on vegetable cowpea has been characterized through rolling circle amplification. The genomic components (DNA A and DNA B) were cloned and sequenced. Nucleotide sequence analysis of DNA A (MT671430) and DNA B (MT671431) component had > 98% identity toward the mungbean yellow mosaic India virus (MYMIV) reported previously from India on various legumes. In phylogenetic analysis, study isolate shared common ancestry with MYMIV isolates of India, Pakistan and Nepal infecting legumes. Based on the recombination analysis, this cowpea isolate appears to be evolved through recombination of MYMIV sequences both at DNA A (Major parent: AF481855; Minor parent: AF416742) and DNA B (Major parent: AF416741; Minor parent: MN698281) level. Furthermore, Agrobacterium-based dimeric clone constructs were found highly infectious on cowpea host upon co-inoculation of DNA-A and DNA-B components by producing typical golden mosaic symptoms 42 days post-inoculation. Upon inoculation of these agro-infectious clones, vegetable cowpea germplasm lines were categorized as resistant, moderately resistant and susceptible to golden mosaic disease.

引用

页数：9

共 27 条

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