Programming Cell Adhesion for On-Chip Sequential Boolean Logic Functions

被引：105

作者：

Qu, Xiangmeng ^{[1
]}

Wang, Shaopeng ^{[2
,3
]}

Ge, Zhilei ^{[2
,3
]}

Wang, Jianbang ^{[2
,3
]}

Yao, Guangbao ^{[2
,3
]}

Li, Jiang ^{[2
,3
]}

Zuo, Xiaolei ^{[2
,3
]}

Shi, Jiye ^{[4
]}

Song, Shiping ^{[2
,3
]}

Wang, Lihua ^{[2
,3
]}

Li, Li ^{[1
]}

Pei, Hao ^{[1
]}

Fan, Chunhai ^{[2
,3
]}

机构：

[1] East China Normal Univ, Shanghai Key Lab Green Chem & Chem Proc, Sch Chem & Mol Engn, 500 Dongchuan Rd, Shanghai 200241, Peoples R China

[2] Chinese Acad Sci, Shanghai Inst Appl Phys, Div Phys Biol, Shanghai Synchrotron Radiat Facil, Shanghai 201800, Peoples R China

[3] Chinese Acad Sci, Shanghai Inst Appl Phys, Bioimaging Ctr, Shanghai Synchrotron Radiat Facil, Shanghai 201800, Peoples R China

[4] Univ Oxford, Kellogg Coll, Oxford OX2 6PN, England

来源：

JOURNAL OF THE AMERICAN CHEMICAL SOCIETY | 2017年 / 139卷 / 30期

基金：

中国博士后科学基金;

关键词：

STRAND DISPLACEMENT CASCADES; DNA NANOSTRUCTURES; COMPUTATION; MICROENVIRONMENTS; AMPLIFICATION; MONOLAYERS; DELIVERY; ROBOTS; ARRAY; ECM;

D O I：

10.1021/jacs.7b04040

中图分类号：

O6 [化学];

学科分类号：

0703 ;

摘要：

Programmable remodelling of cell surfaces enables high-precision regulation of cell behavior. In this work, we developed in vitro constructed DNA-based chemical reaction networks (CRNs) to program on-chip cell adhesion: We found that the RGD-functionalized DNA CRNs are entirely noninvasive when interfaced with the fluidic mosaic membrane of living cells. DNA toehold with different lengths could tunably alter the release kinetics of cells, which shows rapid release in minutes with the use of a 6-base toehold. We further demonstrated the realization of Boolean logic functions by using DNA strand displacement reactions, which include multi-input and sequential cell logic gates (AND, OR, XOR, and AND OR). This study provides a highly generic tool for self organization of biological systems.

引用

页码：10176 / 10179

页数：4

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