Effect of of the anticoagulant, storage time and temperature of blood samples on the concentrations of 27 multiplex assayed cytokines - Consequences for defining reference values in healthy humans

被引:75
作者
Henno, Linda Torrissen [1 ,2 ]
Storjord, Min [1 ,4 ]
Christiansen, Dorte [1 ]
Bergseth, Grete [1 ]
Ludviksen, Judith Krey [1 ]
Fure, Hilde [1 ]
Barene, Svein [3 ]
Waage-Nielsen, Erik [4 ,5 ,6 ]
Mollnes, Tom E. [1 ,4 ,7 ,8 ,9 ]
Brekke, Ole-Lars [1 ,4 ]
机构
[1] Nordland Hosp, Dept Lab Med, Res Lab, Bodo, Norway
[2] Nordland Hosp, Dept Obstet & Gynecol, Bodo, Norway
[3] Inland Norway Univ Appl Sci, Fac Publ Hlth, Elverum, Norway
[4] UiT Arctic Univ Norway, KG Jebsen TREC, Inst Clin Med, Tromso, Norway
[5] Nordland Hosp, Dept Anaesthesiol, Bodo, Norway
[6] North Univ, Bodo, Norway
[7] Univ Oslo, Oslo Univ Hosp, Inst Immunol, Oslo, Norway
[8] Univ Oslo, KG Jebsen IRC, Oslo, Norway
[9] Norwegian Univ Sci & Technol, Ctr Mol Inflammat Res, Trondheim, Norway
关键词
Anticoagulant; Complement; Cytokines; Lepirudin; Plasma; TUMOR-NECROSIS-FACTOR; BIOMATERIAL SURFACES; PERIPHERAL-BLOOD; COMPLEMENT; PLASMA; ACTIVATION; EXPRESSION; INHIBITION; PROFILES; THROMBIN;
D O I
10.1016/j.cyto.2017.05.014
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
Cytokines are potentially useful biomarkers of sepsis and other inflammatory conditions. Many cytokines can be released by leukocytes and platelets after sampling. The sampling and processing techniques are consequently critically important to measure the in vivo levels. We therefore examined the effects of four different anticoagulants, EDTA, citrate, lepirudin, heparin compared to serum, on the levels of 27 different cytokines. The effects of storage temperature, freezing and thawing on the plasma cytokines were examined. Cytokines were analysed using a multiplex immunoassay. The cytokine levels in serum were significantly higher compared with plasma, consistent with release of cytokines in vitro during coagulation. In general, the lowest values for all cytokines were found in EDTA samples, stored on crushed ice, centrifuged within 4 h and thereafter stored at - 80 degrees C. MCP-1 and MIP-1 beta levels were highest in heparin plasma and storage of blood for up to 4 h at room temperature significantly increased the interleukin (IL)-2, IL-6, IL-8, IFN-gamma and GM-CSF levels in EDTA plasma, indicating post-sampling release. In contrast, the IP-10 levels were unaffected by sample storage at both temperatures. Our results indicate that the cytokines were more stable in plasma than in whole blood after sampling. Thus, cytokines should be analysed in EDTA plasma samples stored on ice and centrifuged within 4 h. Based on these data, the reference ranges of 27 cytokines in EDTA plasma in 162 healthy human donors were calculated.
引用
收藏
页码:86 / 95
页数:10
相关论文
共 35 条
[1]   Effect of anticoagulants on multiplexed measurement of cytokine/chemokines in healthy subjects [J].
Biancotto, Angelique ;
Feng, Xingmin ;
Langweiler, Marc ;
Young, Neal S. ;
McCoy, J. Philip .
CYTOKINE, 2012, 60 (02) :438-446
[2]   Combined inhibition of complement and CD14 abolish E-coli-induced cytokine-, chemokine- and growth factor-synthesis in human whole blood [J].
Brekke, Ole-Lars ;
Christiansen, Dorte ;
Fure, Hilde ;
Pharo, Anne ;
Fung, Michael ;
Riesenfeld, Johan ;
Mollnes, Tom Eirik .
MOLECULAR IMMUNOLOGY, 2008, 45 (14) :3804-3813
[3]   Different profiles of cytokine expression during mild and severe acute pancreatitis [J].
Dambrauskas, Zilvinas ;
Giese, Nathalia ;
Gulbinas, Antanas ;
Giese, Thomas ;
Berberat, Pascal O. ;
Pundzius, Juozas ;
Barauskas, Giedrius ;
Friess, Helmut .
WORLD JOURNAL OF GASTROENTEROLOGY, 2010, 16 (15) :1845-1853
[4]   Innate immunity activation on biomaterial surfaces: A mechanistic model and coping strategies [J].
Ekdahl, Kristina N. ;
Lambris, John D. ;
Elwing, Hans ;
Ricklin, Daniel ;
Nilsson, Per H. ;
Teramura, Yuji ;
Nicholls, Ian A. ;
Nilsson, Bo .
ADVANCED DRUG DELIVERY REVIEWS, 2011, 63 (12) :1042-1050
[5]   Thrombosis as an intravascular effector of innate immunity [J].
Engelmann, Bernd ;
Massberg, Steffen .
NATURE REVIEWS IMMUNOLOGY, 2013, 13 (01) :34-45
[6]  
Engstad CS, 1997, THROMB HAEMOSTASIS, V77, P690
[7]   Effects of sample handling on the stability of interleukin 6, tumour necrosis factor-α and leptin [J].
Flower, L ;
Ahuja, RH ;
Humphries, SE ;
Mohamed-Ali, V .
CYTOKINE, 2000, 12 (11) :1712-1716
[8]   Plasma cytokine profiles in systemic sclerosis: associations with autoantibody subsets and clinical manifestations [J].
Gourh, Pravitt ;
Arnett, Frank C. ;
Assassi, Shervin ;
Tan, Filemon K. ;
Huang, Mei ;
Diekman, Laura ;
Mayes, Maureen D. ;
Reveille, John D. ;
Agarwal, Sandeep K. .
ARTHRITIS RESEARCH & THERAPY, 2009, 11 (05)
[9]   Essential role for Ca2+ in regulation of IL-1β secretion by P2X7 nucleotide receptor in monocytes, macrophages, and HEK-293 cells [J].
Gudipaty, L ;
Munetz, J ;
Verhoef, PA ;
Dubyak, GR .
AMERICAN JOURNAL OF PHYSIOLOGY-CELL PHYSIOLOGY, 2003, 285 (02) :C286-C299
[10]   Heparin binds to lipopolysaccharide (LPS)-binding protein, facilitates the transfer of LPS to CD14, and enhances LPS-induced activation of peripheral blood monocytes [J].
Heinzelmann, M ;
Bosshart, H .
JOURNAL OF IMMUNOLOGY, 2005, 174 (04) :2280-2287