Delivery and characterization of sub-8fs laser pulses at the imaging plane of a two-photon microscope

被引:3
作者
Pestov, Dmitry [1 ]
Xu, Bingwei [1 ]
Li, Haowen [1 ]
Dantus, Marcos [1 ]
机构
[1] Biophoton Solut, E Lansing, MI 48823 USA
来源
MULTIPHOTON MICROSCOPY IN THE BIOMEDICAL SCIENCES XI | 2011年 / 7903卷
关键词
ultrafast; two-photon; imaging; microscopy; pulse compression; pulse characterization; MULTIPHOTON INTRAPULSE INTERFERENCE; SPECTRAL PHASE; COMPENSATION;
D O I
10.1117/12.889223
中图分类号
R318 [生物医学工程];
学科分类号
0831 ;
摘要
We demonstrate a modular and versatile experimental setup that enables straightforward compression ( and then shaping) of ultrashort laser pulses at the imaging plane of a two-photon microscope. A commercially available pulse shaper is used in conjunction with a commercially available broadband Ti:Sapphire oscillator to produce sub-8fs pulses at the focus of a high-numerical-aperture objective. Automated adaptive pulse compression, based on multiphoton intrapulse interference phase scan (MIIPS), is verified in situ by shaper assisted interferometric autocorrelation. Two-photon excited fluorescence image of a mouse kidney slide is obtained to confirm microscopic sectioning capabilities.
引用
收藏
页数:4
相关论文
共 8 条
[1]   Interference without an interferometer: a different approach to measuring, compressing, and shaping ultrashort laser pulses [J].
Coello, Yves ;
Lozovoy, Vadim V. ;
Gunaratne, Tissa C. ;
Xu, Bingwei ;
Borukhovich, Ian ;
Tseng, Chien-hung ;
Weinacht, Thomas ;
Dantus, Marcos .
JOURNAL OF THE OPTICAL SOCIETY OF AMERICA B-OPTICAL PHYSICS, 2008, 25 (06) :A140-A150
[2]   Nonlinear Optical Microscopy and Spectroscopy Employing Octave Spanning Pulses [J].
Isobe, Keisuke ;
Suda, Akira ;
Tanaka, Masahiro ;
Hashimoto, Hiroshi ;
Kannari, Fumihiko ;
Kawano, Hiroyuki ;
Mizuno, Hideaki ;
Miyawaki, Atsushi ;
Midorikawa, Katsumi .
IEEE JOURNAL OF SELECTED TOPICS IN QUANTUM ELECTRONICS, 2010, 16 (04) :767-780
[3]   Direct measurement of spectral phase for ultrashort laser pulses [J].
Lozovoy, Vadim V. ;
Xu, Bingwei ;
Coello, Yves ;
Dantus, Marcos .
OPTICS EXPRESS, 2008, 16 (02) :592-597
[4]   Multiphoton intrapulse interference. IV. Ultrashort laser pulse spectral phase characterization and compensation [J].
Lozovoy, VV ;
Pastirk, I ;
Dantus, M .
OPTICS LETTERS, 2004, 29 (07) :775-777
[5]   Theory of double-chirped mirrors [J].
Matuschek, N ;
Kartner, FX ;
Keller, U .
IEEE JOURNAL OF SELECTED TOPICS IN QUANTUM ELECTRONICS, 1998, 4 (02) :197-208
[6]   Multiple Independent Comb Shaping (MICS): Phase-only generation of optical pulse sequences [J].
Pestov, Dmitry ;
Lozovoy, Vadim V. ;
Dantus, Marcos .
OPTICS EXPRESS, 2009, 17 (16) :14351-14361
[7]   Femtosecond pulse shaping using spatial light modulators [J].
Weiner, AM .
REVIEW OF SCIENTIFIC INSTRUMENTS, 2000, 71 (05) :1929-1960
[8]   Quantitative investigation of the multiphoton intrapulse interference phase scan method for simultaneous phase measurement and compensation of femtosecond laser pulses [J].
Xu, BW ;
Gunn, JM ;
Dela Cruz, JM ;
Lozovoy, VV ;
Dantus, M .
JOURNAL OF THE OPTICAL SOCIETY OF AMERICA B-OPTICAL PHYSICS, 2006, 23 (04) :750-759