Plasmonic Nanoprobes for Stimulated Emission Depletion Nanoscopy

被引：32

作者：

Cortes, Emiliano ^{[1
]}

Huidobro, Paloma A. ^{[1
]}

Sinclair, Hugo G. ^{[2
,7
]}

Guldbrand, Stina ^{[2
]}

Peveler, William J. ^{[3
]}

Davies, Timothy ^{[4
]}

Parrinello, Simona ^{[4
]}

Gorlitz, Frederik ^{[2
]}

Dunsby, Chris ^{[2
,5
]}

Neil, Mark A. A. ^{[2
]}

Sivan, Yonatan ^{[6
]}

Parkin, Ivan P. ^{[3
]}

French, Paul M. W. ^{[2
]}

Maier, Stefan A. ^{[1
]}

机构：

[1] Imperial Coll London, Expt Solid State Grp, Dept Phys, London SW7 2AZ, England

[2] Imperial Coll London, Photon Grp, Dept Phys, London SW7 2AZ, England

[3] UCL, Dept Chem, 20 Gordon St, London WC1H 0AJ, England

[4] Imperial Coll London, MRC Clin Sci Ctr CSC, Du Cane Rd, London W12 0NN, England

[5] Imperial Coll London, Ctr Pathol, Du Cane Rd, London W12 0NN, England

[6] Ben Gurion Univ Negev, Unit Electroopt Engn, IL-8410501 Beer Sheva, Israel

[7] UCL, Inst Struct & Mol Biol, Darwin Bldg,Gower St, London WC1E 6BT, England

来源：

ACS NANO | 2016年 / 10卷 / 11期

基金：

英国工程与自然科学研究理事会;

关键词：

STED nanoscopy; nanorods; super resolution; plasmonic nanoparticles; bioimaging; SEMICONDUCTOR QUANTUM DOTS; STED MICROSCOPY; FLUORESCENCE MICROSCOPY; RADIATIVE PROPERTIES; RESOLUTION; GOLD; NANOPARTICLES; SURFACE; CHEMISTRY; BREAKING;

D O I：

10.1021/acsnano.6b06361

中图分类号：

O6 [化学];

学科分类号：

0703 ;

摘要：

Plasmonic nanoparticles influence the absorption and emission processes of nearby emitters due to local enhancements of the illuminating radiation and the photonic density of states. Here, we use the plasmon resonance of metal nanoparticles in order to enhance the stimulated depletion of excited molecules for super-resolved nanoscopy. We demonstrate stimulated emission depletion (STED) nanoscopy with gold nanorods with a long axis of only 26 nm and a width of 8 nm. These particles provide an enhancement of up to 50% of the resolution compared to fluorescent-only probes without plasmonic components irradiated with the same depletion power. The nanoparticle-assisted STED probes reported here represent a similar to 2 x 10(3) reduction in probe volume compared to previously used nanoparticles. Finally, we demonstrate their application toward plasmon-assisted STED cellular imaging at low-depletion powers, and we also discuss their current limitations.

引用

页码：10454 / 10461

页数：8

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