Transgenic Zebrafish Recapitulating tbx16 Gene Early Developmental Expression

被引：13

作者：

Wells, Simon ^{[1
]}

Nornes, Svanhild ^{[1
]}

Lardelli, Michael ^{[1
]}

机构：

[1] Univ Adelaide, Dept Mol Biosci, Special Res Ctr Mol Genet Dev, Adelaide, SA, Australia

来源：

PLOS ONE | 2011年 / 6卷 / 06期

基金：

澳大利亚研究理事会;

关键词：

FLUORESCENT PROTEIN EXPRESSION; SPINAL NEURONS; PROMOTER; VERTEBRATES; EVOLUTION; MUTATION; ELEMENTS; EMBRYOS; MARKS;

D O I：

10.1371/journal.pone.0021559

中图分类号：

O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

We describe the creation of a transgenic zebrafish expressing GFP driven by a 7.5 kb promoter region of the tbx16 gene. This promoter segment is sufficient to recapitulate early embryonic expression of endogenous tbx16 in the presomitic mesoderm, the polster and, subsequently, in the hatching gland. Expression of GFP in the transgenic lines later in development diverges to some extent from endogenous tbx16 expression with the serendipitous result that one line expresses GFP specifically in commissural primary ascending (CoPA) interneurons of the developing spinal cord. Using this line we demonstrate that the gene mafba (valentino) is expressed in CoPA interneurons.

引用

页数：6