A novel near-infrared fluorescence off-on probe for imaging hypoxia and nitroreductase in cells and in vivo

被引：35

作者：

Zhang, Yu ^{[1
]}

Zhang, Xiao-Fan ^{[2
]}

Chen, Qian ^{[1
]}

Cao, Xiao-Qun ^{[1
]}

Shen, Shi-Li ^{[1
]}

机构：

[1] Shandong First Med Univ & Shandong Acad Med Sci, Sch Chem & Pharmaceut Engn, Inst Opt Funct Mat Biomed Imaging, Tai An 271016, Shandong, Peoples R China

[2] Taian Ctr Food & Drug Control, Tai An 271000, Shandong, Peoples R China

来源：

SENSORS AND ACTUATORS B-CHEMICAL | 2022年 / 353卷

基金：

中国国家自然科学基金;

关键词：

Fluorescent probe; Hypoxia; Mitochondria; In vivo imaging; DESIGN; GLUTATHIONE; CYSTEINE;

D O I：

10.1016/j.snb.2021.131145

中图分类号：

O65 [分析化学];

学科分类号：

070302 ; 081704 ;

摘要：

Nitroreductase (NTR) is a highly expressed endogenous enzyme in hypoxic tumor cells, which is associated with the hypoxic degree in tumors. Therefore, it is particularly important to develop low-toxicity and high-sensitivity fluorescent probes for NTR. Herein, we report an NTR-activated near-infrared probe with good selectivity and sensitivity. More importantly, the background fluorescence signal is effectively reduced due to the quenching effect of nitro group as well as the spironolactone (ring-closed) form of rhodamine moiety. Upon reaction with NTR, the probe exhibits a remarkable fluorescence off-on response at 740 nm (about 32-fold intensity enhancement), with an extremely low detection limit of 1.09 ng/mL. Moreover, the probe is able to target mitochondria. With this probe, we have achieved the visualization of NTR overexpression in hypoxic cancer cells, as well as in hypoxic tumors in vivo as revealed in the tumor-bearing mouse model. By using this probe, some essential information may be revealed about hypoxia-relevant diseases.

引用

页数：10

共 33 条

[1] Mitochondrial Nitroreductase Activity Enables Selective Imaging and Therapeutic Targeting [J].