Prevalence, virulence gene distribution and genetic diversity of Arcobacter in food samples in Germany

被引：34
作者：
Lehmann, Daniel [1 ]
Alter, Thomas [1 ]
Lehmann, Laura [1 ]
Uherkova, Simona [2 ]
Seidler, Tassilo [3 ]
Goelz, Greta [1 ]
机构：
[1] Free Univ Berlin, Inst Food Hyg, D-14163 Berlin, Germany
[2] Vet & Pharmaceut Univ Brno, Fac Vet Hyg & Ecol, Brno, Czech Republic
[3] Beuth Univ Appl Sci Berlin, Dept Life Sci & Technol, Berlin, Germany
来源：
BERLINER UND MUNCHENER TIERARZTLICHE WOCHENSCHRIFT | 2015年 / 128卷 / 3-4期
关键词：
Arcobacter; food; prevalence; genotype; virulence genes; CAMPYLOBACTER-JEJUNI; ESCHERICHIA-COLI; RETAIL MARKETS; MULTIPLEX PCR; BUTZLERI; IDENTIFICATION; CARCASSES; PRODUCTS; POULTRY; CELLS;
D O I：
10.2376/0005-9366-128-163
中图分类号：
S85 [动物医学（兽医学）];
学科分类号：
0906 ;
摘要：
This study was carried out to determine the prevalence of Arcobacter spp. in food samples in Germany. In addition, the presence of putative virulence genes and the genetic diversity was tested for Arcobacter (A.) butzleri strains isolated during this study. The prevalence of Arcobacter spp:was 34% in fish meat, 26.8% in poultry meat and 2% in minced meat (beef and pork). All investigated A. butzleri isolates carried the genes cadF, ciaB, cj1349, mviN and pldA. The gene tlyA was detectable in 97.5% of the strains. Lower detection rates were observed for hecA (47.5%), hecB (45%), iroE (40%) and irgA (35%). Genotyping by ERIC-PCR demonstrated a high genetic diversity of A. butzleri strains from different foods. In conclusion, this study shows that about one third of fish meat and poultry meat samples contained Arcobacter spp. These data highlight the need to strengthen our effort to elucidate the importance of Arcobacter on veterinary public health.
引用
收藏
页码：163 / 168
页数：6
相关论文
共 39 条
  • [21] An Outbreak of Foodborne Illness Among Attendees of a Wedding Reception in Wisconsin Likely Caused by Arcobacter butzleri
    Lappi, Victoria
    Archer, John R.
    Cebelinski, Elizabeth
    Leano, Fe
    Besser, John M.
    Klos, Rachel F.
    Medus, Carlota
    Smith, Kirk E.
    Fitzgerald, Collette
    Davis, Jeffrey P.
    [J]. FOODBORNE PATHOGENS AND DISEASE, 2013, 10 (03) : 250 - 255
  • [22] Higher Water Temperature and Incubation under Aerobic and Microaerobic Conditions Increase the Recovery and Diversity of Arcobacter spp. from Shellfish
    Levican, Arturo
    Collado, Luis
    Yustes, Clara
    Aguilar, Carme
    Jose Figueras, Maria
    [J]. APPLIED AND ENVIRONMENTAL MICROBIOLOGY, 2014, 80 (01) : 385 - 391
  • [23] Adherence to and Invasion of Human Intestinal Cells by Arcobacter Species and Their Virulence Genotypes
    Levican, Arturo
    Alkeskas, Aldukali
    Guenter, Claudia
    Forsythe, Stephen J.
    Jose Figueras, Maria
    [J]. APPLIED AND ENVIRONMENTAL MICROBIOLOGY, 2013, 79 (16) : 4951 - 4957
  • [24] Meinersmann RJ, 2002, INT J SYST EVOL MICR, V52, P1789, DOI 10.1099/00207713-52-5-1789
  • [25] Identification of the vibrio cholerae enterobactin receptors VctA and IrgA: IrgA is not required for virulence
    Mey, AR
    Wyckoff, EE
    Oglesby, AG
    Rab, E
    Taylor, RK
    Payne, SM
    [J]. INFECTION AND IMMUNITY, 2002, 70 (07) : 3419 - 3426
  • [26] The Complete Genome Sequence and Analysis of the Epsilonproteobacterium Arcobacter butzleri
    Miller, William G.
    Parker, Craig T.
    Rubenfield, Marc
    Mendz, George L.
    Woesten, Marc M. S. M.
    Ussery, David W.
    Stolz, John F.
    Binnewies, Tim T.
    Hallin, Peter F.
    Wang, Guilin
    Malek, Joel A.
    Rogosin, Andrea
    Stanker, Larry H.
    Mandrell, Robert E.
    [J]. PLOS ONE, 2007, 2 (12):
  • [27] Prevalence and Genetic Diversity of Arcobacter in Food Products in the North of Spain
    Nieva-Echevarria, Barbara
    Martinez-Malaxetxebarria, Irati
    Girbau, Cecilia
    Alonso, Rodrigo
    Fernandez-Astorga, Aurora
    [J]. JOURNAL OF FOOD PROTECTION, 2013, 76 (08) : 1447 - 1450
  • [28] Expression of the Escherichia coli IrgA homolog adhesin is regulated by the ferric uptake regulation protein
    Rashid, Rebecca A.
    Tarr, Phillip I.
    Moseley, Steve L.
    [J]. MICROBIAL PATHOGENESIS, 2006, 41 (06) : 207 - 217
  • [29] Isolation of Arcobacter butzleri from ground water
    Rice, EW
    Rodgers, MR
    Wesley, IV
    Johnson, CH
    Tanner, SA
    [J]. LETTERS IN APPLIED MICROBIOLOGY, 1999, 28 (01) : 31 - 35
  • [30] Isolation and characterisation of Arcobacter butzleri from meat
    Rivas, L
    Fegan, N
    Vanderlinde, P
    [J]. INTERNATIONAL JOURNAL OF FOOD MICROBIOLOGY, 2004, 91 (01) : 31 - 41
1234