Cloning and characterization of rice HMGB1 gene

We isolated a 918 bp long full-length rice HMGB1 cDNA, which has an open reading frame of 471 bp encoding 157 amino acids, with a central domain of high sequence similarity to the HMG-box domain of other plant HMGB I proteins. RNA gel blot analysis indicated that rice HMGB1 gene is constitutively expressed in various tissues and organs. Southern hybridization and sequence analyses suggested that a single copy of the HMGB1 gene composed of seven exons and six introns exists in rice. We have also cloned a 1755 bp long 5' flanking region of the rice HMGB1 gene, which can be regarded as its promoter. 5' deletion analysis of this promoter indicated that positive cis-elements residing between - 1400 and - 1115 are important to enhance quantitative expression, whereas negative cis-elements between - 1755 and - 1400 and between - 1115 and - 351 inhibit expression. (C) 2003 Elsevier Science B.V. All rights reserved.