Construction and analysis of a suppression subtractive hybridization library of regeneration-related genes in soybean

被引:1
作者
Sun, J. [1 ,2 ]
Li, J. [1 ]
Liu, M. [1 ]
Zhang, B. B. [1 ]
Li, D. M. [1 ]
Wang, M. [1 ]
Zhang, C. [1 ]
Li, W. B. [1 ]
Su, A. Y. [3 ]
Wu, X. X. [1 ]
机构
[1] Northeast Agr Univ, Coll Agron, Key Lab Soybean Biol Chinese, Minist Educ, Harbin, Peoples R China
[2] Heilongjiang Acad Agr Sci, Berries Res Inst, Suiling, Heilongjiang, Peoples R China
[3] Northeast Agr Univ, Coll Resource & Environm, Harbin, Peoples R China
基金
黑龙江省自然科学基金;
关键词
Soybean; Suppression subtractive hybridization (SSH); cDNA library; Soybean regeneration; SHOOT REGENERATION; RESPONSE REGULATORS; SIGNAL-TRANSDUCTION; ARABIDOPSIS; CYTOKININS; METABOLISM; PLANTS;
D O I
10.4238/2015.January.30.20
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
The development of a genetic transformation system is needed to address the problem of the low efficiency associated with soybean regeneration. To contribute to the enhancement of the soybean regenerative capacity, we explored the developmental mechanisms of soybean regeneration at the molecular level using a suppression subtractive hybridization cDNA library constructed from cotyledonary nodes of soybean cultivar DN50. A total of 918 positive clones were identified and screened, with most inserted fragments ranging from 100 to 750 bp. Of these, 411 differentially expressed functional expressed sequence tags were identified and annotated based on their similarity to orthologs and paralogs detected in GenBank using the nucleotide and translated nucleotide Basic Local Alignment Search Tools. Functional analysis revealed that the associated genes were involved in signal transduction, synthesis, and metabolism of macromolecules, glucose and protein synthesis and metabolism, light and leaf morphogenesis, regulation of apoptosis, cell defense, cell wall differentiation, and a variety of hormone and cytokinin-mediated signaling pathways. The information uncovered in our study should serve as a foundation for the establishment of an efficient and stable genetic transformation system for soybean regeneration.
引用
收藏
页码:763 / 773
页数:11
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