Engineering of Plants for Efficient Production of Therapeutics

被引：24

作者：

Sethi, Lini ^{[1
,2
]}

Kumari, Khushbu ^{[1
,2
]}

Dey, Nrisingha ^{[1
]}

机构：

[1] Inst Life Sci, Div Plant & Microbial Biotechnol, NALCO Sq, Bhubaneswar 751023, Odisha, India

[2] NCR Delhi, Natl Capital Reg Biotech Sci Cluster, Reg Ctr Biotechnol, Faridabad 121001, Haryana, India

来源：

MOLECULAR BIOTECHNOLOGY | 2021年 / 63卷 / 12期

关键词：

Plant expression system; Edible vaccine; Subcellular targeting; Post-translational modification; Glycoengineering; Protein purification; HIGH-LEVEL EXPRESSION; TRANSGENIC PLANTS; RECOMBINANT PROTEINS; SYNTHETIC PROMOTERS; EDIBLE VACCINES; DOSE-ESCALATION; IMMUNOGENICITY; VIRUS; PURIFICATION; ANTIBODIES;

D O I：

10.1007/s12033-021-00381-0

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Plants are becoming useful platforms for recombinant protein production at present time. With the advancement of efficient molecular tools of genomics, proteomics, plants are now being used as a biofactory for production of different life saving therapeutics. Plant-based biofactory is an established production system with the benefits of cost-effectiveness, high scalability, rapid production, enabling post-translational modification, and being devoid of harmful pathogens contamination. This review introduces the main challenges faced by plant expression system: post-translational modifications, protein stability, biosafety concern and regulation. It also summarizes essential factors to be considered in engineering plants, including plant expression system, promoter, post-translational modification, codon optimization, and fusion tags, protein stabilization and purification, subcellular targeting, and making vaccines in an edible way. This review will be beneficial and informative to scholars and readers in the field of plant biotechnology.

引用

页码：1125 / 1137

页数：13

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