POLYGONUM BISTORTA L. HERBA ET FLORES. POLYPHENOLS PROFILE, ANTIOXIDANT PROPERTIES AND CYTOTOXIC EFFECT ON MURINE FIBROBLAST CELL LINE NIH3T3

This work aimed to study the polyphenols content, the antioxidant activity and the potential cytotoxic effects on fibroblast murine cells NIH3T3 of an ethanolic extract (70%, v/v) obtained from the aerial part (herba et flores) of Polygonum bistorta L. (Polygonaceae family). The tested extract was assessed for its total phenols content: 1.15 mg gallic acid equivalents (GAE) per 1 mL extract and showed numerous flavonoids compounds such as quercetin, kaempferol and luteolin derivates, as well as caffeic and chlorogenic phenolic acids. The chemiluminescence (CL) tests indicated a high antioxidant capacity (IC50 = 0.57 mu g GAE/mL); superior to gallic acid (IC50 = 0.85 mu g/mL) and rutin (IC50 = 2.54 mu g/mL), used as reference compounds. The cytotoxicity test demonstrated that, at concentrations over 25 mu g GAE/mL extract, the ethanolic extracts from the aerial part of Polygonum bistorta plant species have a significant toxicity on mouse embryonic fibroblast NIH3T3 cells.