Production, optimization and characterization of polyhydroxybutyrate, a biodegradable plastic by Bacillus spp.

被引：71

作者：

Bhagowati, Pabitra ^{[1
]}

Pradhan, Shreema ^{[1
]}

Dash, Hirak R. ^{[1
]}

Das, Surajit ^{[1
]}

机构：

[1] Natl Inst Technol, Dept Life Sci, Lab Environm Microbiol & Ecol LEnME, Rourkela, India

来源：

BIOSCIENCE BIOTECHNOLOGY AND BIOCHEMISTRY | 2015年 / 79卷 / 09期

关键词：

fluorescence activated cell sorter (FACS); poly-beta-hydroxybutyrate; melting enthalpy; FTIR; Bacillus; RALSTONIA-EUTROPHA; SYNTHESIS GENES; BIOSYNTHESIS; BACTERIA; PHB; POLYHYDROXYALKANOATES; 3-HYDROXYBUTYRATE; IDENTIFICATION; MEGATERIUM; CARBON;

D O I：

10.1080/09168451.2015.1034651

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Poly-beta-hydroxybutyrate (PHB) is the intracellular lipid reserve accumulated by many bacteria. The most potent terrestrial bacterium Bacillus cereus SE-1 showed more PHB accumulating cells (22.1 and 40% after 48 and 72h) than that of the marine Bacillus sp. CS-605 (5 and 33% after 48 and 72h). Both the isolates harbored phbB gene and the characteristics C=O peak was observed in the extracted PHB by Fourier transformed infrared spectroscopy analysis. Maltose was found to be the most suitable carbon source for the accumulation of PHB in B. cereus SE-1. The extracted PHB sample from B. cereus SE-1 was blended with a thermoplastic starch (TS) and an increased thermoplasticity and decreased crystallinity were observed after blending in comparison to the standard PHB. The melting temperature (T-m), melting enthalpy ( increment Hf), and crystallinity (X-c) of the blended PHB sample were found to be 109.4 degrees C, 64.58J/g, and 44.23%, respectively.

引用

页码：1454 / 1463

页数：10

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