The inhibitory effects of Geranium thunbergii on interferon-γ- and LPS-induced inflammatory responses are mediated by Nrf2 activation

被引：24

作者：

Choi, Hee-Jin ^{[1
,2
,3
]}

Choi, Hee-Jung ^{[2
,3
]}

Park, Mi-Ju ^{[2
,3
]}

Lee, Ji-Yeon ^{[2
,3
]}

Jeong, Seung-Il ^{[4
]}

Lee, Seongoo ^{[5
]}

Kim, Kyun Ha ^{[2
,3
]}

Joo, Myungsoo ^{[2
,3
]}

Jeong, Han-Sol ^{[2
,3
]}

Kim, Jai-Eun ^{[1
]}

Ha, Ki-Tae ^{[2
,3
]}

机构：

[1] Dongguk Univ, Coll Korean Med, Dept Pathol, Ilsan 410773, Gyeonggi Do, South Korea

[2] Pusan Natl Univ, Sch Korean Med, Dept Korean Med Sci, Yangsan 626770, Gyeongsangnam D, South Korea

[3] Pusan Natl Univ, Korean Med Res Ctr Hlth Aging, Yangsan 626770, Gyeongsangnam D, South Korea

[4] Jeonju Biomat Inst, Jeonju, South Korea

[5] Sangji Univ, Coll Korean Med, Dept Korean Pathol, Wonju, Gangwon, South Korea

来源：

INTERNATIONAL JOURNAL OF MOLECULAR MEDICINE | 2015年 / 35卷 / 05期

基金：

新加坡国家研究基金会;

关键词：

Geranium thunbergii; inflammation; interferon-gamma; lipopolysaccharide; nuclear factor erythroid 2-related factor 2; NF-KAPPA-B; OXIDATIVE STRESS; NUCLEAR-FACTOR; PATHWAY; CELLS; CONSTITUENTS; MACROPHAGES; PROTECTION; INDUCTION; EXTRACT;

D O I：

10.3892/ijmm.2015.2128

中图分类号：

R-3 [医学研究方法]; R3 [基础医学];

学科分类号：

1001 ;

摘要：

Geranium thunbergii Sieb. et Zucc. (GT; which belongs to the Geraniaceae family) has been used as a traditional medicine in East Asia for the treatment of inflammatory diseases, including arthritis and diarrhea. However, the underlying mechanisms of the anti-inflammatory effects of GT remain poorly understood. In the present study, we examined the mechanisms responsible for the anti-inflammatory activity of GT in macrophages. The results revealed that GT significantly inhibited the lipopolysaccharide (LPS)- and interferon-gamma (IFN-gamma)-induced expression of pro-inflammatory genes, such as inducible nitric oxide synthase, tumor necrosis factor-a and interleukin-1 beta, as shown by RT-PCR. However, the inhibitory effects of GT on LPS- and IFN-gamma-induced inflammation were associated with an enhanced nuclear factor erythroid 2-related factor 2 (Nrf2) activity, but not with the suppression of nuclear factor (NF)-kappa B activity, as shown by western blot analysis. In addition, in bone marrow-derived macrophages (BMDM) isolated from Nrf2 knockout mice, GT did not exert any inhibitory effect on the LPS- and IFN-gamma-induced inflammation. Taken together, our findings indicate that the anti-inflammatory effects of GT may be associated with the activation of Nrf2, an anti-inflammatory transcription factor.

引用

页码：1237 / 1245

页数：9

共 45 条

[41] Keap1-Nrf2 signaling pathway: Mechanisms of regulation and role in protection of cells against toxicity caused by xenobiotics and electrophiles [J].