Induction of tubulogenesis of microvascular endothelial cells by basic fibroblast growth factor from human SN12C renal cancer cells

被引：8

作者：

Emoto, A

Nakagawa, M

Wakabayashi, Y

Hanada, T

Naito, S

Nomura, Y

机构：

[1] OITA MED UNIV,DEPT UROL & NEUROSURG,OITA 87955,JAPAN

[2] KYUSHU UNIV,SCH MED,DEPT UROL,FUKUOKA 812,JAPAN

来源：

JOURNAL OF UROLOGY | 1997年 / 157卷 / 02期

关键词：

tumor angiogenesis; basic fibroblast growth factor; renal cell carcinoma;

D O I：

10.1016/S0022-5347(01)65253-4

中图分类号：

R5 [内科学]; R69 [泌尿科学（泌尿生殖系疾病）];

学科分类号：

1002 ; 100201 ;

摘要：

Purpose: To investigate the role of basic fibroblast growth factor (b-FGF) in angiogenesis in renal cell carcinoma. Materials and Methods: We examined b-FGF expression in 25 patients with renal cell carcinoma (RCC) by reverse transcriptase polymerase chain reaction (RT-PCR). We measured the tube formation by human omental microvascular endothelial (HOME) cells induced by b-FGF in a three-dimensional collagen gel in the absence or presence of SN12C human renal cancer cells by means of computer-image analysis. Results: RT-PCR analysis detected b-FGF m-RNA in 20 (80%) of 25 patients with RCC. The tube formation of HOME cells was increased in the presence of SN1BC cells overexpressing b-FGF mRNA, induced by exogenous b-FGF in a dose dependent manner and completely inhibited by anti-b-FGF antibody. Conclusions: Basic-FGF may develop tumor angiogenesis by inducing microvessel tubulogenesis in patients with RCC.

引用

页码：699 / 703

页数：5

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