Induction of tubulogenesis of microvascular endothelial cells by basic fibroblast growth factor from human SN12C renal cancer cells

被引：8
作者：
Emoto, A
Nakagawa, M
Wakabayashi, Y
Hanada, T
Naito, S
Nomura, Y
机构：
[1] OITA MED UNIV,DEPT UROL & NEUROSURG,OITA 87955,JAPAN
[2] KYUSHU UNIV,SCH MED,DEPT UROL,FUKUOKA 812,JAPAN
来源：
JOURNAL OF UROLOGY | 1997年 / 157卷 / 02期
关键词：
tumor angiogenesis; basic fibroblast growth factor; renal cell carcinoma;
D O I：
10.1016/S0022-5347(01)65253-4
中图分类号：
R5 [内科学]; R69 [泌尿科学（泌尿生殖系疾病）];
学科分类号：
1002 ; 100201 ;
摘要：
Purpose: To investigate the role of basic fibroblast growth factor (b-FGF) in angiogenesis in renal cell carcinoma. Materials and Methods: We examined b-FGF expression in 25 patients with renal cell carcinoma (RCC) by reverse transcriptase polymerase chain reaction (RT-PCR). We measured the tube formation by human omental microvascular endothelial (HOME) cells induced by b-FGF in a three-dimensional collagen gel in the absence or presence of SN12C human renal cancer cells by means of computer-image analysis. Results: RT-PCR analysis detected b-FGF m-RNA in 20 (80%) of 25 patients with RCC. The tube formation of HOME cells was increased in the presence of SN1BC cells overexpressing b-FGF mRNA, induced by exogenous b-FGF in a dose dependent manner and completely inhibited by anti-b-FGF antibody. Conclusions: Basic-FGF may develop tumor angiogenesis by inducing microvessel tubulogenesis in patients with RCC.
引用
收藏
页码：699 / 703
页数：5
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