Functional degeneration of the resistance gene nsv against Melon necrotic spot virus at low temperature

被引：7

作者：

Kido, Kazutaka ^{[2
]}

Mochizuki, Tomofumi ^{[1
]}

Matsuo, Kazutoshi ^{[3
]}

Tanaka, Chika ^{[4
]}

Kubota, Kenji ^{[5
]}

Ohki, Takehiro ^{[1
]}

Tsuda, Shinya ^{[1
]}

机构：

[1] Natl Agr Res Ctr, Tsukuba, Ibaraki 3058666, Japan

[2] Yokohamaueki Co Ltd, Kanagawa 2328587, Japan

[3] Nagasaki Agr & Forestry Expt Stn, Nagasaki 8540063, Japan

[4] Chiba Prefectural Agr Res Ctr, So Prefectural Hot Inst, Chiba 2940014, Japan

[5] Kyushu Okinawa Reg, Natl Agr Res Ctr, Kumamoto 8611192, Japan

来源：

EUROPEAN JOURNAL OF PLANT PATHOLOGY | 2008年 / 121卷 / 02期

关键词：

cell-to-cell movement; methyl bromide; Olpidium bornovanus; protoplast; recessive resistance; viral replication;

D O I：

10.1007/s10658-007-9249-y

中图分类号：

S3 [农学（农艺学）];

学科分类号：

0901 ;

摘要：

The single recessive gene, nsv, which confers resistance against Melon necrotic spot virus (MNSV), has recently been used to develop virus-resistant melon cultivars in Japan. However, the Chiba isolate of MNSV, a common isolate in Japan, infected resistant cultivars when inoculated melon plants were grown at 15 degrees C. Viral RNAs accumulated in protoplasts from resistant cultivars at both 15 and 20 degrees C. Mechanical inoculation of the cotyledons caused MNSV to spread throughout the leaves at 15 degrees C, but not at 20 degrees C. These results support our novel hypothesis that a temperature-sensitive inactivation of disease resistance genes occurs at the nsv locus in melon cultivars with the resistance gene grown at temperatures below 20 degrees C.

引用

页码：189 / 194

页数：6

共 29 条

[1] Mutations in the eIF(iso)4G translation initiation factor confer high resistance of rice to Rice yellow mottle virus [J].