Background: Esophageal squamous cell carcinoma (ESCC) is one of the most aggressive carcinomas of the gastrointestinal tract. We assessed the relevance of Slug in measuring the invasive potential of ESCC cells in vitro and in vivo in immunodeficient mice. Methods: We utilized RNA interference to knockdown Slug gene expression, and effects on survival and invasive carcinoma were evaluated using a Boyden chamber transwell assay in vitro. We evaluated the effect of Slug siRNA-transfection and Slug cDNA-transfection on E-cadherin and Bcl-2 expression in ESCC cells. A pseudometastatic model of ESCC in immunodeficient mice was used to assess the effects of Slug siRNA transfection on tumor metastasis development. Results: The EC109 cell line was transfected with Slug-siRNA to knockdown Slug expression. The TE13 cell line was transfected with Slug-cDNA to increase Slug expression. EC109 and TE13 cell lines were tested for the expression of apoptosis-related genes bcl-2 and metastasis-related gene E-cadherin identified previously as Slug targets. Bcl-2 expression was increased and E-cadherin was decreased in Slug siRNA-transfected EC109 cells. Bcl-2 expression was increased and E-cadherin was decreased in Slug cDNA-transfected TE13 cells. Invasion of Slug siRNA-transfected EC109 cells was reduced and apoptosis was increased whereas invasion was greater in Slug cDNA-transfected cells. Animals injected with Slug siRNA-transfected EC109 cells exhihited fewer seeded nodes and demonstrated more apoptosis. Conclusions: Slug down-regulation promotes cell apoptosis and decreases invasion capability in vitro and in vivo. Slug inhibition may represent a novel strategy for treatment of metastatic ESCC.
机构:
Zhengzhou Univ, Coll Basic Med Sci, Zhengzhou 450001, Henan, Peoples R ChinaZhengzhou Univ, Coll Basic Med Sci, Zhengzhou 450001, Henan, Peoples R China
Wang, Yuanyuan
Zang, Wenqiao
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Zhengzhou Univ, Coll Basic Med Sci, Zhengzhou 450001, Henan, Peoples R ChinaZhengzhou Univ, Coll Basic Med Sci, Zhengzhou 450001, Henan, Peoples R China
Zang, Wenqiao
Du, Yuwen
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Zhengzhou Univ, Coll Basic Med Sci, Zhengzhou 450001, Henan, Peoples R ChinaZhengzhou Univ, Coll Basic Med Sci, Zhengzhou 450001, Henan, Peoples R China
Du, Yuwen
Ma, Yunyun
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Zhengzhou Univ, Coll Basic Med Sci, Zhengzhou 450001, Henan, Peoples R ChinaZhengzhou Univ, Coll Basic Med Sci, Zhengzhou 450001, Henan, Peoples R China
Ma, Yunyun
Li, Min
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Zhengzhou Univ, Coll Basic Med Sci, Zhengzhou 450001, Henan, Peoples R ChinaZhengzhou Univ, Coll Basic Med Sci, Zhengzhou 450001, Henan, Peoples R China
Li, Min
Li, Ping
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Zhengzhou Univ, Affiliated Hosp 1, Dept Resp Med, Zhengzhou 450052, Henan, Peoples R ChinaZhengzhou Univ, Coll Basic Med Sci, Zhengzhou 450001, Henan, Peoples R China
Li, Ping
Chen, Xudong
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Luohe Med Coll, Dept Histol & Embryol, Luohe 462002, Henan, Peoples R ChinaZhengzhou Univ, Coll Basic Med Sci, Zhengzhou 450001, Henan, Peoples R China
Chen, Xudong
Wang, Tao
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机构:
Henan Univ TCM, Affiliated Hosp 1, Dept Hematotumor, Zhengzhou 450000, Henan, Peoples R ChinaZhengzhou Univ, Coll Basic Med Sci, Zhengzhou 450001, Henan, Peoples R China
Wang, Tao
Dong, Ziming
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Zhengzhou Univ, Coll Basic Med Sci, Zhengzhou 450001, Henan, Peoples R ChinaZhengzhou Univ, Coll Basic Med Sci, Zhengzhou 450001, Henan, Peoples R China
Dong, Ziming
Zhao, Guoqiang
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Zhengzhou Univ, Coll Basic Med Sci, Zhengzhou 450001, Henan, Peoples R ChinaZhengzhou Univ, Coll Basic Med Sci, Zhengzhou 450001, Henan, Peoples R China
机构:
Anhui Med Univ, Affiliated Hosp 1, Dept Med Oncol, Hefei, Anhui, Peoples R ChinaAnhui Med Univ, Affiliated Hosp 1, Dept Med Oncol, Hefei, Anhui, Peoples R China
Zheng, D-N
Zhang, C-J
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Anhui Med Univ, Affiliated Hosp 1, Dept Intervent, Hefei, Anhui, Peoples R ChinaAnhui Med Univ, Affiliated Hosp 1, Dept Med Oncol, Hefei, Anhui, Peoples R China
Zhang, C-J
Sun, G-P
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Anhui Med Univ, Affiliated Hosp 1, Dept Med Oncol, Hefei, Anhui, Peoples R ChinaAnhui Med Univ, Affiliated Hosp 1, Dept Med Oncol, Hefei, Anhui, Peoples R China
机构:
Weihai Municipal Hosp, Dept Stomatol, Weihai, Peoples R ChinaWeihai Municipal Hosp, Dept Stomatol, Weihai, Peoples R China
Xing, H-B
Qiu, H-M
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机构:
Liaocheng Peoples Hosp, Dept Stomatol, Liaocheng, Shandong, Peoples R ChinaWeihai Municipal Hosp, Dept Stomatol, Weihai, Peoples R China
Qiu, H-M
Li, Y.
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Weihai Municipal Hosp, Dept Gynaecol, Weihai, Peoples R ChinaWeihai Municipal Hosp, Dept Stomatol, Weihai, Peoples R China
Li, Y.
Dong, P-F
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Weihai Municipal Hosp, Dept Stomatol, Weihai, Peoples R ChinaWeihai Municipal Hosp, Dept Stomatol, Weihai, Peoples R China
Dong, P-F
Zhu, X-M
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Qingdao Stomatol Hosp, Multidisciplinary Consultat Clin, Qingdao, Shandong, Peoples R ChinaWeihai Municipal Hosp, Dept Stomatol, Weihai, Peoples R China