Differences in optical trapping prompt investigations of Agrobacterium surface characteristics

被引：2

作者：

Buer, CS ^{[1
]}

Gahagan, KT ^{[1
]}

Swartzlander, GA ^{[1
]}

Weathers, PJ ^{[1
]}

机构：

[1] Worcester Polytech Inst, Dept Biol Biotechnol, Worcester, MA 01609 USA

来源：

JOURNAL OF INDUSTRIAL MICROBIOLOGY & BIOTECHNOLOGY | 1998年 / 21卷 / 4-5期

关键词：

Agrobacterium rhizogenes; Agrobacterium tumefaciens; capsule; calcofluor; exopolysaccharide; optical tweezers; rheometry;

D O I：

10.1038/sj.jim.2900573

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

Comparison of the optical trapping efficiency of Agrobacterium rhizogenes and A. tumefaciens strains indicates the A. rhizogenes strain, ATCC 11325, is significantly less efficiently trapped than A. rhizogenes A4, ATCC 15834, and the A. tumefaciens strain LBA4404. Differences were also found in capsule generation, growth media viscosity, and transmission electron microscopy negative staining. These observations imply a difference in surface structure exists. Calcofluor fluorescence suggests the difference involves an exopolysaccharide.

引用

页码：233 / 236

页数：4

共 26 条

[1] FORCES OF A SINGLE-BEAM GRADIENT LASER TRAP ON A DIELECTRIC SPHERE IN THE RAY OPTICS REGIME [J].

ASHKIN, A .

BIOPHYSICAL JOURNAL, 1992, 61 (02) :569-582

[2]

Budavari S., 1996, MERCK INDEX, V12th

[3]

Buer CS, 1998, BIOTECHNOL BIOENG, V60, P348, DOI 10.1002/(SICI)1097-0290(19981105)60:3&lt

[4]

348::AID-BIT11&gt

[5]

3.0.CO

[6]

2-I

[7] COMMON LOCI FOR AGROBACTERIUM-TUMEFACIENS AND RHIZOBIUM-MELILOTI EXOPOLYSACCHARIDE SYNTHESIS AND THEIR ROLES IN PLANT INTERACTIONS [J].

CANGELOSI, GA ;

HUNG, L ;

PUVANESARAJAH, V ;

STACEY, G ;

OZGA, DA ;

LEIGH, JA ;

NESTER, EW .

JOURNAL OF BACTERIOLOGY, 1987, 169 (05) :2086-2091

[8]

DAVEY M, 1994, SYST ASS SPEC, V49, P9

[9] SOLUTION PROPERTIES OF EXOCELLULAR MICROBIAL POLYSACCHARIDES .3. LIGHT-SCATTERING FROM GELLAN AND FROM THE EXOCELLULAR POLYSACCHARIDE OF RHIZOBIUM-TRIFOLII (STRAIN-TA-1) IN THE ORDERED STATE [J].

DENTINI, M ;

COVIELLO, T ;

BURCHARD, W ;

CRESCENZI, V .

MACROMOLECULES, 1988, 21 (11) :3312-3320

[10]

DINER AM, 1987, EUR J FOREST PATHOL, V17, P211

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