Quantitative Determination of Triperpene Saponins and Alkenated-Phenolics from Labisia pumila Using an LC-UV/ELSD Method and Confirmation by LC-ESI-TOF

This study describes the first analytical method for the determination of four triterpene saponins (ardisicrenoside B, ardisiacrispin A, 3-O-alpha-L-rhamnopyranosyl-(1 -> 2)-beta-D-glucopyranosyl-(1 -> 4)-alpha-L-arabinopynanosyl cyclamiretin A and ardisimamilloside H) and three alkenated-phenolics (irisresorcinol, belamcandol B, and demethyl-belamcandaquinone B) from the leaves, leaves/stems, and roots of Labisia pumila using an HPLC-UV-ELSD method. The separation was achieved using a reversed-phase (C-18) column, PDA and ELS detection, and a water/acetonitrile gradient as the mobile phase. The major triterpenoid (ardisiacrispin A) and irisresorcinol com-pounds were detected at a concentration as low as 10.0 and 0.2 mu g/mL, respectively. Analysis of various samples showed considerable variation of 0.11-2.46% for the major triterpenoid compound, ardisiacrispin A. LC-mass spectrometry method coupled with electrospray ionization (ESI) is described for the identification of compounds in plant samples. This method involved the use of the [ M + Na](+) and [ M + NH4](+) ions for compounds 1-4 in the positive ion mode with extractive ion chromatogram (EIC).