2D MoSe2@PVP nanosheets with multi-enzyme activity alleviate the acute pancreatitis via scavenging the reactive oxygen and nitrogen species

Acute pancreatitis (AP) is a disease with high mortality which could cause an inflammatory responses such as edema and necrosis of the pancreatic tissue. Studies have proved that the excessive produced reactive oxygen species (ROS) and reactive nitrogen species (RNS) play an important role in the pathogenesis of AP. Although progresses have been made for ROS and RNS elimination using artificial enzymes, biocompatible nanomaterials with advanced multi-enzymatic activities have been rarely studied. In this study, we constructed a simple and efficient method to fabricate a two-dimensional (2D) MoSe2@PVP nanosheets (NSs) as an artificial enzyme to treat oxidative stress-related diseases. Both in vitro and in vivo experiments proved that MoSe2@PVP NSs have good biocompatibility. Interestingly, the MoSe2@PVP NSs simultaneously showed the thermal durable peroxidase, catalase, superoxide dismutase, and glutathione peroxidase mimicking capacities, which endowed the material with good ROS and RNS scavenging abilities. The MoSe2@PVP NSs were engaged in treating the AP in an animal model, which led to a significant down-regulation of serum levels of amylase, IL-6, IL-1 beta, and TNF-alpha. Further H&E staining of pancreatic tissue validated the AP therapeutic effect of MoSe2@PVP NSs. We hope that our research will facilitate the development of multifunctional artificial enzymes for the treating of oxidative stress-related diseases.