miR-1236-3p suppresses the migration and invasion by targeting KLF8 in lung adenocarcinoma A549 cells

被引：45

作者：

Bian, Tingting ^{[1
]}

Jiang, Daishan ^{[2
]}

Liu, Jian ^{[3
]}

Yuan, Xiaopeng ^{[4
]}

Feng, Jia ^{[1
]}

Li, Qian ^{[1
]}

Zhang, Qing ^{[1
]}

Li, Xiaoli ^{[1
]}

Liu, Yifei ^{[1
]}

Zhang, Jianguo ^{[1
]}

机构：

[1] Nantong Univ, Affiliated Hosp, Dept Pathol, Nantong, Jiangsu, Peoples R China

[2] Nantong Univ, Affiliated Hosp, Dept Emergency Med, Nantong, Jiangsu, Peoples R China

[3] Nantong Univ, Affiliated Hosp, Dept Chemotherapy, Nantong, Jiangsu, Peoples R China

[4] Nantong Univ, Affiliated Tumor Hosp, Nantong Tumor Hosp, Dept Radiat Oncol, Nantong, Jiangsu, Peoples R China

来源：

BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS | 2017年 / 492卷 / 03期

关键词：

miR-1236-3p; KLF8; Epithelial mesenchymal transition(EMT); Migration; Invasion; EPITHELIAL-MESENCHYMAL TRANSITION; FOCAL ADHESION KINASE; CANCER METASTASIS; HEPATOCELLULAR-CARCINOMA; COLORECTAL-CANCER; POOR-PROGNOSIS; GASTRIC-CANCER; MIR-200; FAMILY; MICRORNAS; TRANSCRIPTION;

D O I：

10.1016/j.bbrc.2017.08.074

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

MicroRNAs (miRNAs) have a great effect on regulating tumor cell migration, invasion, proliferation and prognosis. However, the mechanism of miR-1236-3p on regulating carcinogenesis is still unknown. In this study, the expression of miR-1236-3p was lower in lung adenocarcinoma tissues than that in adjacent normal tissue. In lung adenocarcinoma A549 cell line, miR-1236-3p decreased ability of cell invasion and migration, furthermore, we show that KLF8 is targeted by miR-1236-3p, and expression of miR-1236-3p is negatively correlated with KLF8. Additionally, miR-1236-3p suppressed the expression of KLF8 and EMT (epithelial mesenchymal transition)-related genes. Overexpression of KLF8 can promote EMT -related genes at protein level. In conclusion, our results support the fact that miR-1236-3p acts as a tumor inhibitor in lung adenocarcinoma by suppressing the activity of KLF8, and it may play a critical role in the diagnosis and treatment of lung cancer. (C) 2017 Published by Elsevier Inc.

引用

页码：461 / 467

页数：7

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