miR-1236-3p suppresses the migration and invasion by targeting KLF8 in lung adenocarcinoma A549 cells

被引：44
作者：
Bian, Tingting [1 ]
Jiang, Daishan [2 ]
Liu, Jian [3 ]
Yuan, Xiaopeng [4 ]
Feng, Jia [1 ]
Li, Qian [1 ]
Zhang, Qing [1 ]
Li, Xiaoli [1 ]
Liu, Yifei [1 ]
Zhang, Jianguo [1 ]
机构：
[1] Nantong Univ, Affiliated Hosp, Dept Pathol, Nantong, Jiangsu, Peoples R China
[2] Nantong Univ, Affiliated Hosp, Dept Emergency Med, Nantong, Jiangsu, Peoples R China
[3] Nantong Univ, Affiliated Hosp, Dept Chemotherapy, Nantong, Jiangsu, Peoples R China
[4] Nantong Univ, Affiliated Tumor Hosp, Nantong Tumor Hosp, Dept Radiat Oncol, Nantong, Jiangsu, Peoples R China
来源：
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS | 2017年 / 492卷 / 03期
关键词：
miR-1236-3p; KLF8; Epithelial mesenchymal transition(EMT); Migration; Invasion; EPITHELIAL-MESENCHYMAL TRANSITION; FOCAL ADHESION KINASE; CANCER METASTASIS; HEPATOCELLULAR-CARCINOMA; COLORECTAL-CANCER; POOR-PROGNOSIS; GASTRIC-CANCER; MIR-200; FAMILY; MICRORNAS; TRANSCRIPTION;
D O I：
10.1016/j.bbrc.2017.08.074
中图分类号：
Q5 [生物化学]; Q7 [分子生物学];
学科分类号：
071010 ; 081704 ;
摘要：
MicroRNAs (miRNAs) have a great effect on regulating tumor cell migration, invasion, proliferation and prognosis. However, the mechanism of miR-1236-3p on regulating carcinogenesis is still unknown. In this study, the expression of miR-1236-3p was lower in lung adenocarcinoma tissues than that in adjacent normal tissue. In lung adenocarcinoma A549 cell line, miR-1236-3p decreased ability of cell invasion and migration, furthermore, we show that KLF8 is targeted by miR-1236-3p, and expression of miR-1236-3p is negatively correlated with KLF8. Additionally, miR-1236-3p suppressed the expression of KLF8 and EMT (epithelial mesenchymal transition)-related genes. Overexpression of KLF8 can promote EMT -related genes at protein level. In conclusion, our results support the fact that miR-1236-3p acts as a tumor inhibitor in lung adenocarcinoma by suppressing the activity of KLF8, and it may play a critical role in the diagnosis and treatment of lung cancer. (C) 2017 Published by Elsevier Inc.
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收藏
页码：461 / 467
页数：7
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