3D characterization of the forces in optical traps based on counter-propagating beams shaped by a spatial light modulator

被引:0
作者
Kristensen, Martin V. [1 ]
Lindballe, Thue B. [2 ]
Kylling, Anton P. [3 ]
Palima, Darwin Z. [4 ]
Gluckstad, Jesper [4 ]
Keiding, Soren R. [1 ,3 ]
Stapelfeldt, Henrik [1 ,3 ]
机构
[1] Aarhus Univ, Interdisciplinary Nanosci Ctr, DK-8000 Aarhus C, Denmark
[2] Aarhus Univ, Dept Phys & Astron, DK-8000 Aarhus C, Denmark
[3] Aarhus Univ, Dept Chem, DK-8000 Aarhus C, Denmark
[4] Tech Univ Denmark, DTU Fotonik, Dept Photon Engn, DK-2800 Lyngby, Denmark
来源
OPTICAL TRAPPING AND OPTICAL MICROMANIPULATION VII | 2010年 / 7762卷
关键词
optical trap; trapping forces; counter-propagating beams geometry; asymmetric trap; noise; TWEEZERS; MICROMANIPULATION;
D O I
10.1117/12.866479
中图分类号
O43 [光学];
学科分类号
070207 ; 0803 ;
摘要
An experimental characterization of the 3D forces, acting on a trapped polystyrene bead in a counter-propagating beam geometry, is reported. Using a single optical trap with a large working distance (in the BioPhotonics Workstation), we simultaneously measure the transverse and longitudinal trapping force constants. Two different methods were used: The Drag force method and the Equipartition method. We show that the counter-propagating beams traps are simple harmonic for small displacements. The force constants reveal a transverse asymmetry as kappa(-) = 9.7 pN/mu m and kappa(+) = 11.3 pN/mu m (at a total laser power of 2x35 mW) for displacements in opposite directions. The Equipartition method is limited by mechanical noise and is shown to be applicable only when the total laser power in a single 10 mu m counter-propagating trap is below 2x20 mW.
引用
收藏
页数:12
相关论文
empty
未找到相关数据