Enantiomer separation by pressure-supported electrochromatography using capillaries packed with a permethyl-β-cyclodextrin stationary phase

被引：90

作者：

Wistuba, D ^{[1
]}

Czesla, H ^{[1
]}

Roeder, M ^{[1
]}

Schurig, V ^{[1
]}

机构：

[1] Univ Tubingen, Inst Organ Chem, D-72076 Tubingen, Germany

来源：

JOURNAL OF CHROMATOGRAPHY A | 1998年 / 815卷 / 02期

关键词：

enantiomer separation; electrochromatography; chiral stationary phases; LC; barbiturates;

D O I：

10.1016/S0021-9673(98)00472-5

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

Efficient enantiomer separation by pressure-assisted, micro-packed capillary electrochromatography (CEC) has been carried out using a permethyl-beta-cyclodextrin-modified silica support (PM-beta-CD-silica). When comparing this method with micro-packed-high-performance liquid chromatography in the single-column-mode, CEC displays higher column efficiencies (about three times higher theoretical plate numbers at comparable elution times). The pressure support (about 10 bar), applied to avoid bubble formation, has a negligible influence on elution times in CEC. The influence of the type and composition of organic modifiers is described. (C) 1998 Elsevier Science B.V. All rights reserved.

引用

页码：183 / 188

页数：6

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