Highly efficient chemoenzymatic synthesis of β1-3-linked galactosides

被引：70

作者：

Yu, Hai ^{[1
]}

Thon, Vireak ^{[1
]}

Lau, Kam ^{[1
]}

Cai, Li ^{[2
,3
]}

Chen, Yi ^{[1
]}

Mu, Shengmao ^{[1
]}

Li, Yanhong ^{[1
]}

Wang, Peng George ^{[2
,3
]}

Chen, Xi ^{[1
]}

机构：

[1] Univ Calif Davis, Dept Chem, Davis, CA 95616 USA

[2] Ohio State Univ, Dept Biochem, Columbus, OH 43210 USA

[3] Ohio State Univ, Dept Chem, Columbus, OH 43210 USA

来源：

CHEMICAL COMMUNICATIONS | 2010年 / 46卷 / 40期

关键词：

ANTIGEN GLOBO-H; N-BIOSE-I; BIFIDOBACTERIUM-LONGUM; ENZYMATIC-SYNTHESIS; HUMAN-MILK; PHOSPHORYLASE; OLIGOSACCHARIDES;

D O I：

10.1039/c0cc02850a

中图分类号：

O6 [化学];

学科分类号：

0703 ;

摘要：

A novel D-galactosyl-beta 1-3-N-acetyl-D-hexosamine phosphorylase cloned from Bifidobacterium infantis (BiGalHexNAcP) was used with a recombinant E. coli K-12 galactokinase (GalK) for efficient one-pot two-enzyme synthesis of T-antigens, galacto-N-biose (Gal beta 1-3GalNAc), lacto-N-biose (Gal beta 1-3GlcNAc), and their derivatives.

引用

收藏

页码：7507 / 7509

页数：3

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