Proliferation of Peripheral Blood Lymphocytes and Mesenchymal Stromal Cells Derived from Wharton's Jelly in Mixed and Membrane-Separated Cultures

被引：4

作者：

Poltavtsev, A. M. ^{[1
]}

Poltavtseva, R. A. ^{[2
]}

Yushina, M. N. ^{[2
]}

Pavlovich, S. V. ^{[2
]}

Svirshchevskaya, E. V. ^{[3
]}

机构：

[1] Russian Acad Sci, Inst Appl Mech, Moscow, Russia

[2] Minist Hlth Russian Federat, VI Kulakov Res Ctr Obstet Gynecol & Perinatol, Moscow, Russia

[3] Russian Acad Sci, MM Shemyakin & Yu Ovchinnikov Inst Bioorgan Che, Moscow, Russia

来源：

BULLETIN OF EXPERIMENTAL BIOLOGY AND MEDICINE | 2017年 / 163卷 / 04期

基金：

俄罗斯科学基金会;

关键词：

mesenchymal stromal cells; peripheral blood lymphocytes; Transwell cultures; multicellular spheroids; cell cycle; STEM-CELLS; IN-VITRO; IMMUNOMODULATORY PROPERTIES; ACTIVATED LYMPHOCYTES; KILLER-CELLS; INHIBIT;

D O I：

10.1007/s10517-017-3846-3

中图分类号：

R-3 [医学研究方法]; R3 [基础医学];

学科分类号：

1001 ;

摘要：

We studied the effect of mesenchymal stromal cells on proliferation of CFSE-stained T cells in mixed and membrane-separated (Transwell) cultures and in 3D culture of mesenchymal stromal cells from Wharton's jelly. The interaction of mesenchymal stromal cells with mitogen-activated peripheral blood lymphocytes from an allogeneic donor was followed by suppression of T-cell proliferation in a wide range of cell proportions. Culturing in the Transwell system showed the absence of suppression assessed by the fraction of proliferating cells and by the cell cycle analysis. In 3D cultures, contact interaction of mesenchymal stromal cells and lymphocytes was demonstrated that led to accumulation of G2/M phase lymphocytes and G0/G1 phase mesenchymal stromal cells. The suppressive effect of mesenchymal stromal cells from Wharton's jelly is mediated by two mechanisms. The effects are realized within 6 days, which suggests that the therapeutic effects of mesenchymal stromal cells persist until their complete elimination from the body.

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页码：542 / 549

页数：8

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