A heat-inducible Bacillus subtilis bacteriophage Φ105 expression system for the production of the protective antigen of Bacillus anthracis

被引：0

作者：

Baillie, LWJ ^{[1
]}

Moore, P

McBride, BW

机构：

[1] CBD Porton Down, Def Evaluat & Res Agcy, Salisbury SP4 0JQ, Wilts, England

[2] Publ Hlth Lab Serv, Ctr Appl Microbiol & Res, Salisbury SP4 0JG, Wilts, England

来源：

FEMS MICROBIOLOGY LETTERS | 1998年 / 163卷 / 01期

关键词：

Bacillus subtilis; prophage; expression; vaccine;

D O I：

暂无

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

The protective antigen of Bacillus anthracis is the major protective immunogen in the current human vaccine. A heat-inducible protective antigen expression system was constructed based on a derivative of Bacillus subtilis phage Phi 105. The recombinant protein produced by this system protected immunised animals against challenge with spores of B. anthracis. Gene instability and protease activity of the host strain contributed to the low level of recoverable protein in culture supernatant (similar to 2 mg l(-1)). (C) 1998 Federation of European Microbiological Societies. Published by Elsevier Science B.V. All rights reserved.

引用

页码：43 / 47

页数：5

共 14 条

[1] REQUIREMENTS FOR TRANSFORMATION IN BACILLUS SUBTILIS [J].

ANAGNOSTOPOULOS, C ;

SPIZIZEN, J .

JOURNAL OF BACTERIOLOGY, 1961, 81 (05) :741-&

[2]

BAILLIE LWJ, 1996, P INT WORKSH ANTHR 1, V87, P133

[3]

BELTON FC, 1954, BRIT J EXP PATHOL, V35, P144

[4]

Bron S., 1990, Molecular biological methods for Bacillus, P75

[5]

CUTTING SM, 1990, MOL BIOL METHOD BACI, P26

[6]

Errington J., 1990, Molecular Biological Methods for Bacillus, P175

[7] CLONING AND EXPRESSION OF THE BACILLUS-ANTHRACIS PROTECTIVE ANTIGEN GENE IN BACILLUS-SUBTILIS [J].

IVINS, BE ;

WELKOS, SL .

INFECTION AND IMMUNITY, 1986, 54 (02) :537-542

[8]

Leppla S.H., 1991, SOURCEBOOK BACTERIAL, P277

[9]

Maniatis T., 1982, MOL CLONING A LAB MA

[10]

MCBRIDE BW, 1998, IN PRESS VACCINE

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