A Commensal Gone Bad: Complete Genome Sequence of the Prototypical Enterotoxigenic Escherichia coli Strain H10407

被引：142

作者：

Crossman, Lisa C. ^{[2
]}

Chaudhuri, Roy R. ^{[3
]}

Beatson, Scott A. ^{[4
]}

Wells, Timothy J. ^{[1
]}

Desvaux, Mickael ^{[1
]}

Cunningham, Adam F. ^{[1
]}

Petty, Nicola K. ^{[2
]}

Mahon, Vivienne ^{[6
]}

Brinkley, Carl ^{[7
]}

Hobman, Jon L. ^{[9
]}

Savarino, Stephen J. ^{[8
]}

Turner, Susan M. ^{[1
]}

Pallen, Mark J. ^{[5
]}

Penn, Charles W. ^{[5
]}

Parkhill, Julian ^{[2
]}

Turner, A. Keith ^{[2
]}

Johnson, Timothy J. ^{[10
]}

Thomson, Nicholas R. ^{[2
]}

Smith, Stephen G. J. ^{[6
]}

Henderson, Ian R. ^{[1
]}

机构：

[1] Univ Birmingham, Sch Immun & Infect, Birmingham B15 2TT, W Midlands, England

[2] Wellcome Trust Sanger Inst, Cambridge, England

[3] Univ Cambridge, Dept Vet Med, Cambridge, England

[4] Univ Queensland, Sch Chem & Mol Biosci, Brisbane, Qld, Australia

[5] Univ Birmingham, Sch Biosci, Birmingham B15 2TT, W Midlands, England

[6] Trinity Coll Dublin, Sch Med, Dept Clin Microbiol, Dublin, Ireland

[7] Walter Reed Army Inst Res, Dept Enter Infect, Silver Spring, MD USA

[8] Enter Dis Dept, Silver Spring, MD USA

[9] Univ Nottingham, Sch Biosci, Loughborough, England

[10] Univ Minnesota, Dept Vet & Biomed Sci, St Paul, MN 55108 USA

来源：

JOURNAL OF BACTERIOLOGY | 2010年 / 192卷 / 21期

基金：

英国生物技术与生命科学研究理事会;

关键词：

EPITHELIAL-CELL INVASION; HEAT-LABILE ENTEROTOXIN; MOLECULAR CHARACTERIZATION; PLASMID STABILITY; GENE-CLUSTER; PROTEIN; IDENTIFICATION; SECRETION; COLONIZATION; VIRULENCE;

D O I：

10.1128/JB.00710-10

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

In most cases, Escherichia coli exists as a harmless commensal organism, but it may on occasion cause intestinal and/or extraintestinal disease. Enterotoxigenic E. coli (ETEC) is the predominant cause of E. coli-mediated diarrhea in the developing world and is responsible for a significant portion of pediatric deaths. In this study, we determined the complete genomic sequence of E. coli H10407, a prototypical strain of enterotoxigenic E. coli, which reproducibly elicits diarrhea in human volunteer studies. We performed genomic and phylogenetic comparisons with other E. coli strains, revealing that the chromosome is closely related to that of the nonpathogenic commensal strain E. coli HS and to those of the laboratory strains E. coli K-12 and C. Furthermore, these analyses demonstrated that there were no chromosomally encoded factors unique to any sequenced ETEC strains. Comparison of the E. coli H10407 plasmids with those from several ETEC strains revealed that the plasmids had a mosaic structure but that several loci were conserved among ETEC strains. This study provides a genetic context for the vast amount of experimental and epidemiological data that have been published.

引用

页码：5822 / 5831

页数：10

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