Crude Extracts of Agaricus brasiliensis Induce Apoptosis in Human Oral Cancer CAL 27 Cells through a Mitochondria-dependent Pathway

被引：0

作者：

Fan, Ming-Jen ^{[1
]}

Lin, Yu-Chin ^{[1
]}

Shih, Hsin-Der ^{[2
]}

Yang, Jai-Sing ^{[3
]}

Liu, Kuo-Ching ^{[5
]}

Yang, Su-Tso ^{[7
,8
]}

Lin, Chien-Yih ^{[1
]}

Wu, Rick Sai-Chuen ^{[9
]}

Yu, Chun-Shu ^{[6
]}

Ko, Yang-Ching ^{[10
]}

Chung, Jing-Gung ^{[1
,4
]}

机构：

[1] Asia Univ, Dept Biotechnol, Taichung 413, Taiwan

[2] Council Agr, Taiwan Agr Res Inst, Plant Pathol Div, Taichung 413, Taiwan

[3] China Med Univ, Dept Pharmacol, Taichung 404, Taiwan

[4] China Med Univ, Dept Biol Sci & Technol, Taichung 404, Taiwan

[5] China Med Univ, Dept Med Lab Sci & Biotechnol, Taichung 404, Taiwan

[6] China Med Univ, Sch Pharm, Taichung 404, Taiwan

[7] China Med Univ, Sch Chinese Med, Taichung 404, Taiwan

[8] China Med Univ, China Med Univ Hosp, Dept Radiol, Taichung 404, Taiwan

[9] China Med Univ Hosp, Dept Anesthesiol, Taichung 404, Taiwan

[10] St Martin De Porres Hosp, Dept Internal Med, Div Pulm & Crit Care Med, Chiayi 600, Taiwan

来源：

IN VIVO | 2011年 / 25卷 / 03期

关键词：

Agaricus brasiliensis; human oral cancer CAL 27 cells; apoptosis; mitochondria-dependent pathway; GRAFTED TUMOR SYSTEM; BLAZEI-MURILL; IN-VITRO; HUMAN-PAPILLOMAVIRUS; ANTITUMOR-ACTIVITY; BEARING MICE; BETA-GLUCAN; MCF7; CELLS; FRUIT BODY; MUSHROOM;

D O I：

暂无

中图分类号：

R-3 [医学研究方法]; R3 [基础医学];

学科分类号：

1001 ;

摘要：

In Taiwan, oral cancer is the fourth leading cause of male cancer mortality, and is still increasing. The Basiodiomycete, Agaricus brasiliensis Mural (ABM) is a dietary mushroom and has been known for its immune-enhancing, antitumor, antioxidation, antiviral and anti-mutagenesis functions. However, the exact anticancer mechanisms of ABM on human oral cancer cells are still unclear. In the present study, we investigated the effects of 50% ethanol crude extracts and hot water extracts of ABM on oral cancer CAL 27 cells. We observed that 0.9 mg/ml and 0.7 mg/ml of ABM 50% ethanol crude extracts and hot water, respectively, caused morphological changes and significantly reduced cell viability after 48-h treatment. The results showed that both extracts of ABM inhibited cell proliferation, increased the Ca2+ release, reduced the mitochondria membrane potential (Delta Psi m), and caused cell cycle arrest in the G(0)/G(1) I phase, which contributed to apoptosis. Additionally, ABM induced DNA fragmentation, a characteristic of apoptosis and the expressions of apoptosis-related proteins, including apoptosis-inducing factor, cytochrome c, and caspase-3, were increased. Overall, we demonstrated that 50% ethanol crude extract and hot water extracts of ABM were able to induce apoptotic cell death in CAL 27 cells via the release of cytochrome c from mitochondria into the cytoplasm and activation of caspase-3 in vitro.

引用

页码：355 / 366

页数：12

共 56 条

[1] Ruthenium red, inhibitor of mitochondrial Ca2+ uniporter, inhibits curcumin-induced apoptosis via the prevention of intracellular Ca2+ depletion and cytochrome c release [J].