Laser based disruption of bacillus spores on a microchip

被引：0

作者：

Hofmann, O ^{[1
]}

Murray, K ^{[1
]}

Wilkinson, AS ^{[1
]}

Cox, T ^{[1
]}

Manz, A ^{[1
]}

机构：

[1] Univ London Imperial Coll Sci & Technol, Ctr Analyt Sci, London SW7 2AZ, England

来源：

Micro Total Analysis Systems 2004, Vol 2 | 2005年 / 297期

关键词：

microchip; spore disruption; extraction; PCR;

D O I：

暂无

中图分类号：

O6 [化学];

学科分类号：

0703 ;

摘要：

We report on the development of a laser based spore disruption method. Bacillus globigii spores were mixed with a laser light absorbing matrix and co-crystallized into 200-mu m-wide and 20-mu m-deep nanovials formed in a polydimethylsiloxane (PDMS) target plate. Surface tension effects were exploited to affect up to 125-fold spore enrichment. When the target zones Were illuminated at atmospheric pressure with pulsed UV laser light, a change in spore morphology was observed within seconds. Post illumination PCR analysis suggests the release of endogenous DNA indicative of spore disruption.

引用

页码：258 / 260

页数：3