Challenges and perspectives of the β-galactosidase enzyme

被引：26

作者：

Damin, B. I. S. ^{[1
]}

Kovalski, F. C. ^{[2
]}

Fischer, J. ^{[3
]}

Piccin, J. S. ^{[1
]}

Dettmer, A. ^{[1
]}

机构：

[1] Univ Passo Fundo UPF, Fac Agron & Vet Med FAMV, Postgrad Program Food Sci & Technol PPGCTA, Passo Fundo, RS, Brazil

[2] Univ Passo Fundo UPF, Fac Engn & Architecture FEAR, Chem Engn Course, Passo Fundo, RS, Brazil

[3] Univ Passo Fundo UPF, Inst Exact Sci & Geosci ICEG, Chem Course, Passo Fundo, RS, Brazil

来源：

APPLIED MICROBIOLOGY AND BIOTECHNOLOGY | 2021年 / 105卷 / 13期

关键词：

Supports; Biocatalysis; Immobilization; Enzymatic hydrolysis; KLUYVEROMYCES-LACTIS; LACTOSE HYDROLYSIS; COVALENT IMMOBILIZATION; EFFICIENT IMMOBILIZATION; GLYOXYL-AGAROSE; IONIC-BINDING; CROSS-LINKING; WHEY LACTOSE; CHEESE WHEY; CHITOSAN;

D O I：

10.1007/s00253-021-11423-7

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

The enzyme beta-galactosidase has great potential for application in the food and pharmaceutical industries due to its ability to perform the hydrolysis of lactose, a disaccharide present in milk and in dairy by-products. It can be used in free form, in batch processes, or in immobilized form, which allows continuous operation and provides greater enzymatic stability. The choice of method and support for enzyme immobilization is essential, as the performance of the biocatalyst is strongly influenced by the properties of the material used and by the interaction mechanisms between support and enzyme. Therefore, this review showed the main enzyme immobilization techniques, and the most used supports for the constitution of biocatalysts. Also, materials with the potential for immobilization of beta-galactosidases and the importance of their biotechnological application are presented.

引用

页码：5281 / 5298

页数：18

共 122 条

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