Testicular transcript responses in rare minnow Gobiocypris rarus following different concentrations bisphenol A exposure

Bisphenol A (BPA) is widely spread in the environment. It can cause various reproductive disrupting effects on different organisms, including fish. To investigate the effect of BPA at different concentrations comprehensively, RNA-seq was performed on the testicular mRNA libraries of adult male rare minnow Gobiocypris rarus that exposed to 0, 1, 15 and 225 mu g/L. BPA for 7 days. Meanwhile, biological indicators and sex steroid hormone levels were investigated. Result showed that (1) BPA at all three concentrations affected the expression of genes related to testicular steroid hormone biosynthesis, blood-testis barrier, proteolysis, and lipid transport and metabolism. (2) EPA at 1 mu g/L induced gene expression in renin-angiotensin system pathway and possibly initiate membrane form of estrogen receptor (mER); 1 and 15 mu g/L BPA inhibited tRNA processing-related genes expression; 15 and 225 a BPA decreased hemostasis and blood coagulation-related gene expression. The present study indicated that BPA did influence rare minnow testicular gene expressing, and the effect BPA effects varied with concentration. (C) 2016 Elsevier Ltd. All rights reserved.