Three-dimensional culture systems in cancer research: Focus on tumor spheroid model

被引：674
作者：
Nath, Sritama [1 ]
Devi, Gayathri R. [1 ,2 ]
机构：
[1] Duke Univ, Div Surg Sci, Dept Surg, Sch Med, Durham, NC 27710 USA
[2] Duke Univ, Duke Canc Inst, Womens Canc Program, Sch Med, Durham, NC 27710 USA
来源：
PHARMACOLOGY & THERAPEUTICS | 2016年 / 163卷
关键词：
Tumor emboli; Apoptosis; High-throughput screening; Inflammatory breast cancer; Oxidative stress; Invasion; INFLAMMATORY BREAST-CANCER; MULTICELLULAR SPHEROIDS; EXTRACELLULAR-MATRIX; DRUG-DELIVERY; MICROENVIRONMENTAL REGULATION; CELLULAR SPHEROIDS; MAGNETIC HYDROGELS; PROTEIN EXPRESSION; CELLS; 3D;
D O I：
10.1016/j.pharmthera.2016.03.013
中图分类号：
R9 [药学];
学科分类号：
1007 ;
摘要：
Cancer cells propagated in three-dimensional (3D) culture systems exhibit physiologically relevant cell-cell and cell-matrix interactions, gene expression and signaling pathway profiles, heterogeneity and structural complexity that reflect in vivo tumors. In recent years, development of various 3D models has improved the study of host-tumor interaction and use of high-throughput screening platforms for anti-cancer drug discovery and development. This review attempts to summarize the various 3D culture systems, with an emphasis on,the most well characterized and widely applied model multicellular tumor spheroids. This review also highlights the various techniques to generate tumor spheroids, methods to characterize them, and its applicability in cancer research. (C) 2016 Elseviet Inc. All rights reserved.
引用
收藏
页码：94 / 108
页数：15
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