HMGN2: a novel antimicrobial effector molecule of human mononuclear leukocytes?

被引:35
作者
Feng, Y [1 ]
Huang, N [1 ]
Wu, Q [1 ]
Wang, BY [1 ]
机构
[1] Sichuan Univ, W China Med Ctr, Res Unit Infect & Immun, Chengdu 610041, Sichuan, Peoples R China
关键词
antimicrobial activity; alpha-helical domain; subcellular distribution;
D O I
10.1189/jlb.0505280
中图分类号
Q2 [细胞生物学];
学科分类号
071009 ; 090102 ;
摘要
Leukocytes are a central cellular element of innate-immune defense in mammals. In addition to the generation of toxic oxygen radicals and nitric oxide, leukocytes express and secrete a broad array of antimicrobial proteins and peptides. In the study, an antimicrobial polypeptide was isolated and purified from human peripheral blood mononuclear leukocytes in the presence of interleukin (IL)-2. Microsequencing provided that its N-terminal amino sequence was PKRKAEGDAK, which was identical to high mobility group nucleosomal-binding domain 2 (HMGN2). Mass spectrometric value and Western blot also indicated its individual character of HMGN2. The antimicrobial assays showed that the Escherichia coli-based production of HMGN2 had a potent antimicrobial activity against E. coli ML-35p, Pseudomonas aeruginosa ATCC 27853, and to some extent, against Candida albicans ATCC 10231. The HMGN2 ut-helical domain had the same antimicrobial activity as HMGN2. The immunocytochemistry staining, enzyme-linked immunosorbent assay, and Western blot revealed that HMGN2 was present in the cytoplasm of mononuclear leukocytes and released to the extracellular environment when stimulated with IL-2. These results suggest that HMGN2 would be a novel antimicrobial effector molecule of human mononuclear leukocyte.
引用
收藏
页码:1136 / 1141
页数:6
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