Optimization of a nitrite-dependent anaerobic methane oxidation (n-damo) process by enhancing methane availability

被引:30
作者
Hu, Zhen [1 ]
Ru, Dongyun [1 ]
Wang, Yinan [1 ]
Zhang, Jian [1 ,2 ]
Jiang, Liping [1 ]
Xu, Xinyi [1 ]
Nie, Lichao [3 ]
机构
[1] Shandong Univ, Sch Environm Sci & Engn, Shandong Key Lab Water Pollut Control & Resource, Qingdao 266237, Peoples R China
[2] Shandong Univ, State Key Lab Microbial Technol, Qingdao 266237, Peoples R China
[3] Shandong Univ, Geotech & Struct Engn Res Ctr, Jinan 250061, Shandong, Peoples R China
基金
中国国家自然科学基金;
关键词
Nitrite-dependent anaerobic methane oxidation; Static pressure; Immobilized biological activated carbon; Potential n-damo activity; Abundance; OXIDIZING BACTERIA; ENRICHMENT; PRESSURE; WATER; DENITRIFICATION; MICROORGANISMS; STIMULATION; GROWTH; MODEL;
D O I
10.1016/j.biortech.2018.12.035
中图分类号
S2 [农业工程];
学科分类号
0828 ;
摘要
The nitrite-dependent anaerobic methane oxidation (n-damo) process is believed to have good application prospects, but its development is limited by the low growth rate and low specific activity of n-damo bacteria. In this study, immobilized biologically activated carbon (IC) and high static pressure (HP) were investigated to improve the performance of the n-damo process by enhancing methane availability. Both IC and HP improved the activity of n-damo bacteria, and the highest n-damo specific activity was obtained in the HP-IC system, which reached 47.65 +/- 0.21 mu mol N L-1 g(-1) h(-1). The larger specific surface area of biomass in the HP system was conducive to the transfer of methane, whereas IC increased the methane absorption capacity by approximately 10-fold and facilitated the methane supply to n-damo bacteria. Furthermore, q-PCR analysis revealed that both HP and IC improved the growth rate of n-damo bacteria, while HP could also enrich the diversity of n-damo bacteria.
引用
收藏
页码:101 / 108
页数:8
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