Conjugated anionic PEG-citrate G2 dendrimer with multi-epitopic HIV-1 vaccine candidate enhance the cellular immune responses in mice

被引：17

作者：

Abdoli, Asghar ^{[1
,2
]}

Radmehr, Nina

Bolhassani, Azam ^{[1
]}

Eidi, Akram ^{[2
]}

Mehrbod, Parvaneh ^{[3
]}

Motevalli, Fatemeh ^{[1
]}

Kianmehr, Zahra ^{[4
]}

Chiani, Mohsen ^{[5
]}

Mahdavi, Mehdi ^{[6
]}

Yazdani, Shaghayegh ^{[7
]}

Ardestani, Mehdi Shafiee ^{[8
]}

Kandi, Mohammad Reza ^{[9
]}

Aghasadeghi, Mohammad Reza ^{[1
]}

机构：

[1] Pasteur Inst Iran, Hepatitis & AIDS Dept, Tehran, Iran

[2] Islamic Azad Univ, Fac Basic Sci, Sci & Res Branch, Tehran, Iran

[3] Pasteur Inst Iran, Influenza & Other Resp Viruses Dept, Tehran, Iran

[4] Shahed Univ, Immunoregulat Res Ctr, Tehran, Iran

[5] Pasteur Inst Iran, Dept Pilot Biotechnol, Tehran, Iran

[6] Pasteur Inst Iran, Dept Immunol, Tehran, Iran

[7] Univ Tehran Med Sci, Sch Publ Hlth, Dept Virol, Tehran, Iran

[8] Univ Tehran Med Sci, Fac Pharm, Dept Radiopharm, Tehran, Iran

[9] Univ Tehran, Fac New Sci & Technol, Dept Life Sci Engn, Tehran, Iran

来源：

ARTIFICIAL CELLS NANOMEDICINE AND BIOTECHNOLOGY | 2017年 / 45卷 / 08期

基金：

美国国家科学基金会;

关键词：

Epitope; vaccine; HIV; dendrimer; CpG motif; DNA; NANOPARTICLES; PEPTIDE; DRUG; STRATEGY; DELIVERY; ANTIGEN; BINDING; CELLS; GAG;

D O I：

10.1080/21691401.2017.1290642

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

Multi-epitope vaccines might cause immunity against multiple antigenic targets. Four immunodominant epitopes of HIV-1 genome were used to construct a polytope vaccine, formulated by dendrimer. Two regimens of polytopes mixture with dendrimer were utilized to immunize BALB/c mice. Adjuvants were also used to boost immune responses. The conjugated polytope could arouse significant cellular immune responses (P<0.05) and Th1 response showed higher intensity compared to Th2 (P<0.05). Our study depicted that conjugated dendrimer with multi-epitopic rHIVtop4 would efficiently induce cell-mediated immune responses and might be considered as promising delivery system for vaccines formulation.

引用

页码：1762 / 1768

页数：7

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