HGF suppresses the production of collagen type III and α-SMA induced by TGF-β1 in healing fibroblasts

被引：30
|
作者：
Jiang, Dapeng [1 ]
Jiang, Zhitao [1 ]
Han, Fuyou [1 ]
Zhang, Yubo
Li, Zhaozhu [1 ]
机构：
[1] Harbin Med Coll, Affiliated Hosp 2, Dept Pediat Surg, Harbin 150086, Peoples R China
来源：
EUROPEAN JOURNAL OF APPLIED PHYSIOLOGY | 2008年 / 103卷 / 05期
关键词：
hepatocyte growth factor; transforming growth factor beta (TGF-beta); collagen; alpha-smooth muscle actin (alpha-SMA); medial collateral ligament; myofibroblast;
D O I：
10.1007/s00421-008-0733-7
中图分类号：
Q4 [生理学];
学科分类号：
071003 ;
摘要：
The aim of this study was to examine the effectiveness of HGF in blocking TGF-beta 1-induced collagen III and alpha-smooth muscle actin (alpha-SMA) production in rat healing fibroblasts, fibroblasts were obtained from healing medial collateral ligament (MCL) injury. Cell culture was supplemented with 5 ng/ml of TGF-beta 1 along with increasing doses of HGF (10-40 ng/ml). The productions of collagen III in supernatants culture were assayed by enzyme-linked immunosorbent assay. Expression of alpha-SMA was assessed by Western blot. Treatment with TGF-beta 1 significantly stimulated collagen III and alpha-SMA production in healing fibroblasts. Remarkably, the addition of HGF reduced productions of all components induced by TGF-beta 1 in a dose-dependent manner. This study shows that HGF antagonizes the action of TGF-beta 1 effectively in cultured healing MCL injury fibroblasts. The results provide a cellular and molecular basis for HGF's acting as a therapeutic agent for MCL scar formation and poor healing.
引用
收藏
页码：489 / 493
页数：5
相关论文
共 50 条
  • [1] HGF suppresses the production of collagen type III and α-SMA induced by TGF-β1 in healing fibroblasts
    Dapeng Jiang
    Zhitao Jiang
    Fuyou Han
    Yubo Zhang
    Zhaozhu Li
    European Journal of Applied Physiology, 2008, 103 : 489 - 493
  • [2] FGF2 SUPPRESSES THE TGF-β-INDUCED COLLAGEN AND α-SMA EXPRESSION IN HUMAN LUNG FIBROBLASTS
    Wang, X.
    Tanino, Y.
    Sato, S.
    Ishii, T.
    Inokoshi, Y.
    Nakagawa, N.
    Saito, K.
    Fukuhara, A.
    Nikaido, T.
    Ishida, T.
    Munakata, M.
    RESPIROLOGY, 2011, 16 : 26 - 26
  • [3] Glucose-induced fibronectin and collagen type III expression in renal fibroblasts can occur independent of TGF-β1
    Lam, S
    Verhagen, NAM
    Strutz, F
    Van Der Pijl, JW
    Daha, MR
    Van Kooten, C
    KIDNEY INTERNATIONAL, 2003, 63 (03) : 878 - 888
  • [4] TGF-BETA EFFECT ON COLLAGEN PRODUCTION BY NORMAL AND HGF FIBROBLASTS
    TIPTON, DA
    FITZGERALD, WJ
    ROBINSON, QC
    DABBOUS, MK
    JOURNAL OF DENTAL RESEARCH, 1995, 74 : 202 - 202
  • [5] Ticlopidine inhibits TGF-β1-induced collagen type I expression in cardiac fibroblasts
    Sigel, AV
    Peng, CF
    Kromer, EP
    CIRCULATION, 1998, 98 (17) : 180 - 180
  • [6] Regulation of collagen production by TGF-β2 in bronchial fibroblasts
    Campbell, G. A.
    Harrop, C.
    Gore, R. B.
    Herrick, S. E.
    CLINICAL AND EXPERIMENTAL ALLERGY, 2009, 39 (12): : 1938 - 1938
  • [7] OPC-15161 suppresses the proliferation of Tenon's capsule fibroblasts and the production of type I collagen and fibronectin stimulated by TGF-β1 in vitro
    Saika, S
    Yamanaka, O
    Kawashima, Y
    Ohkawa, K
    Ohnishi, Y
    Ooshima, A
    Kimura, M
    Nakano, Y
    Kao, WWY
    CURRENT EYE RESEARCH, 1998, 17 (09) : 933 - 940
  • [8] Stimulation of collagen production by TGF-β1 during differentiation of cardiac fibroblasts to myofibroblasts
    Petrov, VV
    Lijnen, PJ
    Fagard, RH
    JOURNAL OF HYPERTENSION, 2002, 20 : S29 - S29
  • [9] Stretching-induced collagen type I synthesis in human tendon fibroblasts is mediated by TGF-β1
    Yang, GG
    Crawford, RC
    Wang, JHC
    2003 ADVANCES IN BIOENGINEERING, 2003, : 127 - 128
  • [10] Trichostatin A, a histone deacetylase inhibitor, suppresses collagen synthesis and prevents TGF-β1-induced fibrogenesis in skin fibroblasts
    Rombouts, K
    Niki, T
    Greenwel, P
    Vandermonde, A
    Wielant, A
    Hellemans, K
    De Bleser, P
    Yoshida, M
    Schuppan, D
    Rojkind, M
    Geerts, A
    EXPERIMENTAL CELL RESEARCH, 2002, 278 (02) : 184 - 197
12345