POU-M2 promotes juvenile hormone biosynthesis by directly activating the transcription of juvenile hormone synthetic enzyme genes in Bombyx mori

被引：14

作者：

Cai, Rui ^{[1
]}

Tao, Gang ^{[1
]}

Zhao, Ping ^{[1
,2
]}

Xia, Qingyou ^{[1
,2
]}

He, Huawei ^{[1
,2
,3
]}

Wang, Yejing ^{[1
,2
]}

机构：

[1] Southwest Univ, Biol Sci Res Ctr, State Key Lab Silkworm Genome Biol, Chongqing, Peoples R China

[2] Southwest Univ, Chongqing Engn & Technol Res Ctr Novel Silk Mat, Chongqing Key Lab Sericultural Sci, Chongqing, Peoples R China

[3] Southwest Univ, Chongqing Key Lab Soft atter Mat Chem & Funct Mfg, Chongqing, Peoples R China

来源：

OPEN BIOLOGY | 2022年 / 12卷 / 04期

关键词：

Bombyx mori; POU-M2; juvenile hormone biosynthesis; JHAMT; transcriptional regulation; DOMAIN PROTEIN; DNA-BINDING; EXPRESSION; ECDYSONE; IDENTIFICATION; METAMORPHOSIS; REPRESSION; DRIFTER; REGULATOR; MECHANISM;

D O I：

10.1098/rsob.220031

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Juvenile hormone (JH) plays a key role in preventing larval precocious metamorphosis, maintaining larval state, controlling adult sexual development and promoting insect egg maturation. Genetic studies have shown that POU factor ventral veins lacking regulates JH synthesis to control the timing of insect metamorphosis. However, how POU factor regulates JH synthesis is largely unknown. Here, we found POU-M2 was highly expressed in corpora allata (CA) and specifically localized in the nucleus of CA. The overexpression of POU-M2 promoted the expression of JH synthase genes and kr-h1 and enhanced the activity of JH synthase genes promoter. Further, POU-M2 promoted the transcription of JH acid O-methyltransferase (JHAMT) by directly binding to the key cis-regulatory elements -207, -249 and -453 within the proximal regions of JHAMT promoter. Both the POU domain and homeodomain were vital for the activation of POU-M2 on JHAMT transcription. Our study reveals the mechanism by which POU-M2 regulates JHAMT transcription.

引用

页数：11

共 51 条

[1] POU domain factors in the neuroendocrine system: Lessons from developmental biology provide insights into human disease [J].

Andersen, B ;

Rosenfeld, MG .

ENDOCRINE REVIEWS, 2001, 22 (01) :2-35

[2]

Anderson MG, 1996, DEVELOPMENT, V122, P4169

[3] DRIFTER, A DROSOPHILA POU-DOMAIN TRANSCRIPTION FACTOR, IS REQUIRED FOR CORRECT DIFFERENTIATION AND MIGRATION OF TRACHEAL CELLS AND MIDLINE GLIA [J].

ANDERSON, MG ;

PERKINS, GL ;

CHITTICK, P ;

SHRIGLEY, RJ ;

JOHNSON, WA .

GENES & DEVELOPMENT, 1995, 9 (01) :123-137

[4] Ecdysone signalling and ovarian development in insects: from stem cells to ovarian follicle formation [J].

Belles, Xavier ;

Piulachs, Maria-Dolors .

BIOCHIMICA ET BIOPHYSICA ACTA-GENE REGULATORY MECHANISMS, 2015, 1849 (02) :181-186

[5]

Certel K, 2000, DEVELOPMENT, V127, P3173

[6] The POU Factor Ventral Veins Lacking/Drifter Directs the Timing of Metamorphosis through Ecdysteroid and Juvenile Hormone Signaling [J].

Cheng, CeCe ;

Ko, Amy ;

Chaieb, Leila ;

Koyama, Takashi ;

Sarwar, Prioty ;

Mirth, Christen K. ;

Smith, Wendy A. ;

Suzuki, Yuichiro .

PLOS GENETICS, 2014, 10 (06)

[7] Knockout silkworms reveal a dispensable role for juvenile hormones in holometabolous life cycle [J].

Daimon, Takaaki ;

Uchibori, Miwa ;

Nakao, Hajime ;

Sezutsu, Hideki ;

Shinoda, Tetsuro .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 2015, 112 (31) :E4226-E4235

[8] Precocious Metamorphosis in the Juvenile Hormone-Deficient Mutant of the Silkworm, Bombyx mori [J].

Daimon, Takaaki ;

Kozaki, Toshinori ;

Niwa, Ryusuke ;

Kobayashi, Isao ;

Furuta, Kenjiro ;

Namiki, Toshiki ;

Uchino, Keiro ;

Banno, Yutaka ;

Katsuma, Susumu ;

Tamura, Toshiki ;

Mita, Kazuei ;

Sezutsu, Hideki ;

Nakayama, Masayoshi ;

Itoyama, Kyo ;

Shimada, Toru ;

Shinoda, Tetsuro .

PLOS GENETICS, 2012, 8 (03)

[9] Homeodomain POU and Abd-A proteins regulate the transcription of pupal genes during metamorphosis of the silkworm, Bombyx mori [J].

Deng, Huimin ;

Zhang, Jialing ;

Li, Yong ;

Zheng, Sichun ;

Liu, Lin ;

Huang, Lihua ;

Xu, Wei-Hua ;

Palli, Subba R. ;

Feng, Qili .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 2012, 109 (31) :12598-12603

[10]

FUKUTA M, 1993, J BIOL CHEM, V268, P19471

← 1 2 3 4 5 6 →